Deciphering the contribution of microbial biomass to the properties of dissolved and particulate organic matter in anaerobic digestates

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Fernández-Domínguez, DavidINRAE, Univ. Montpellier, LBE, 102 Avenue des étangs, Narbonne, France (författare)

Deciphering the contribution of microbial biomass to the properties of dissolved and particulate organic matter in anaerobic digestates

Artikel/kapitelEngelska2023

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Elsevier,2023
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LIBRIS-ID:oai:DiVA.org:umu-206368
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-206368URI
https://doi.org/10.1016/j.scitotenv.2023.162882DOI
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-193373URI

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Språk:engelska
Sammanfattning på:engelska

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Funding Agencies|French Occitanie Region [00004786-24001447]; French National Research Institute for Agriculture, Food and Environment (INRAE); Doctoral School GAIA (University of Montpellier for Life and Environmental Sciences, and Technology Sciences); Swedish Energy Agency through the Biogas Solutions Research Center (BSRC) in Linkoping [P2021-90266]
The recalcitrant structures either from substrate or microbial biomass contained in digestates after anaerobic digestion (AD) highly influence digestate valorization. To properly assess the microbial biomass contribution to the digested organic matter (OM), a combination of characterization methods and the use of various substrate types in anaerobic continuous reactors was required. The use of totally biodegradable substrates allowed detecting soluble microbial products via fluorescence spectroscopy at emission wavelengths of 420 and 460 nm while the protein-like signature was enhanced by the whey protein. During reactors' operation, a transfer of complex compounds to the dissolved OM from the particulate OM was observed through fluorescence applied on biochemical fractionation. Consequently, the fluorescence complexity index of the dissolved OM increased from 0.59–0.60 to 1.06–1.07, whereas it decreased inversely for the extractable soluble from the particulate OM from 1.16–1.19 to 0.42–0.54. Accordingly, fluorescence regional integration showed differences among reactors based on visual inspection and orthogonal partial latent structures (OPLS) analysis. Similarly, the impact of the substrate type and operation time on the particulate OM was revealed by 13C nuclear magnetic resonance using OPLS, providing a good model (R2X = 0.93 and Q2 = 0.8) with a clear time-trend. A high signal resonated at ∼30 ppm attributed to CH2-groups in the aliphatic chain of lipid-like structure besides carbohydrates intensities at 60–110 ppm distinguished the reactor fed with whey protein from the other, which was mostly biomass related. Indeed, this latter displayed a higher presence of peptidoglycan (δH/C: 1.6–2.0/20–25 ppm) derived from microbial biomass by 1H-13C heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance. Interestingly, the sample distribution obtained by non-metric multidimensional scaling of bacterial communities resembled the attained using 13C NMR properties, opening new research perspectives. Overall, this study discloses the microbial biomass contribution to digestates composition to improve the OM transformation mechanism knowledge.

Ämnesord och genrebeteckningar

NATURVETENSKAP Biologi Mikrobiologi hsv//swe
NATURAL SCIENCES Biological Sciences Microbiology hsv//eng
NATURVETENSKAP Kemi Analytisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
Digestate
Endogenous compounds
Fluorescence
Microbiology
Nuclear magnetic resonance
Sludge

Biuppslag (personer, institutioner, konferenser, titlar ...)

Shakeri Yekta, SepehrLinköpings universitet,Tema Miljöförändring,Filosofiska fakulteten(Swepub:liu)sepsh83 (författare)
Hedenström, Mattias,1976-Umeå universitet,Kemiska institutionen,Umea Univ, Sweden(Swepub:umu)mashem96 (författare)
Patureau, DominiqueINRAE, Univ. Montpellier, LBE, 102 Avenue des étangs, Narbonne, France (författare)
Jimenez, JulieINRAE, Univ. Montpellier, LBE, 102 Avenue des étangs, Narbonne, France (författare)
INRAE, Univ. Montpellier, LBE, 102 Avenue des étangs, Narbonne, FranceTema Miljöförändring (creator_code:org_t)

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Ingår i:Science of the Total Environment: Elsevier8770048-96971879-1026

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Av författaren/redakt...: Fernández-Domíng ...; Shakeri Yekta, S ...; Hedenström, Matt ...; Patureau, Domini ...; Jimenez, Julie

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NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Mikrobiologi

NATURVETENSKAP: NATURVETENSKAP; och Kemi; och Analytisk kemi

Artiklar i publikationen: Science of the T ...

Av lärosätet: Umeå universitet; Linköpings universitet

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