A microfluidic-based approach to investigate the inflammatory response of macrophages to pristine and drug-loaded nanostructured hydroxyapatite

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Fältnamn	Indikatorer	Metadata
000		05086naa a2200469 4500
001		oai:DiVA.org:uu-480694
003		SwePub
008		220716s2022 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4806942 URI
024	7	a https://doi.org/10.1016/j.mtbio.2022.1003512 DOI
040		a (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Carter, Sarah-Sophia,d 1994-u Uppsala universitet,Institutionen för materialvetenskap,Science for Life Laboratory, SciLifeLab,EMBLA4 aut0 (Swepub:uu)sarca385
245	1 0	a A microfluidic-based approach to investigate the inflammatory response of macrophages to pristine and drug-loaded nanostructured hydroxyapatite
264	1	b Elsevier,c 2022
338		a electronic2 rdacarrier
520		a The in vitro biological characterization of biomaterials is largely based on static cell cultures. However, for highly reactive biomaterials such as calcium-deficient hydroxyapatite (CDHA), this static environment has limitations. Drastic alterations in the ionic composition of the cell culture medium can negatively affect cell behavior, which can lead to misleading results or data that is difficult to interpret. This challenge could be addressed by a microfluidics-based approach (i.e. on-chip), which offers the opportunity to provide a continuous flow of cell culture medium and a potentially more physiologically relevant microenvironment. The aim of this work was to explore microfluidic technology for its potential to characterize CDHA, particularly in the context of inflammation. Two different CDHA substrates (chemically identical, but varying in microstructure) were integrated on-chip and subsequently evaluated. We demonstrated that the on-chip environment can avoid drastic ionic alterations and increase protein sorption, which was reflected in cell studies with RAW 264.7 macrophages. The cells grown on-chip showed a high cell viability and enhanced proliferation compared to cells maintained under static conditions. Whereas no clear differences in the secretion of tumor necrosis factor alpha (TNF-α) were found, variations in cell morphology suggested a more anti-inflammatory environment on-chip. In the second part of this study, the CDHA substrates were loaded with the drug Trolox. We showed that it is possible to characterize drug release on-chip and moreover demonstrated that Trolox affects the TNF-α secretion and morphology of RAW 264.7 cells. Overall, these results highlight the potential of microfluidics to evaluate (bioactive) biomaterials, both in pristine form and when drug-loaded. This is of particular interest for the latter case, as it allows the biological characterization and assessment of drug release to take place under the same dynamic in vitro environment.
650	7	a NATURVETENSKAPx Biologix Biofysik0 (SwePub)106032 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Biophysics0 (SwePub)106032 hsv//eng
653		a Biomaterial
653		a Calcium phosphate cement
653		a Drug release
653		a In vitro
653		a Macrophage
653		a On-chip
653		a Engineering Science with specialization in Biomedical Engineering
653		a Teknisk fysik med inriktning mot medicinsk teknik
700	1	a Atif, Abdul Raouf,d 1996-u Uppsala universitet,Institutionen för materialvetenskap,Science for Life Laboratory, SciLifeLab,EMBLA4 aut0 (Swepub:uu)abdat742
700	1	a Diez-Escudero, Annau Uppsala universitet,Ortopedi,Ortholab4 aut0 (Swepub:uu)anndi833
700	1	a Grape, Majau Uppsala universitet,Institutionen för materialvetenskap,Science for Life Laboratory, SciLifeLab,EMBLA4 aut0 (Swepub:uu)majgr178
700	1	a Ginebra, Maria-Pauu Biomaterials, Biomechanics and Tissue Engineering Group, Departament de Ciència i Enginyeria de Materials, Universitat Politècnica de Catalunya (UPC), 08930, Barcelona, Spain.;Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya, 08930, Barcelona, Spain.;Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology (BIST), Baldiri Reixac 10-12, 08028, Barcelona, Spain.4 aut
700	1	a Tenje, Mariau Uppsala universitet,Institutionen för materialvetenskap,Science for Life Laboratory, SciLifeLab,EMBLA4 aut0 (Swepub:uu)marte323
700	1	a Mestres, Gemma,d 1984-u Uppsala universitet,Institutionen för materialvetenskap,Science for Life Laboratory, SciLifeLab,EMBLA4 aut0 (Swepub:uu)gemme924
710	2	a Uppsala universitetb Institutionen för materialvetenskap4 org
773	0	t Materials Today Biod : Elsevierg 16q 16x 2590-0064
856	4	u https://doi.org/10.1016/j.mtbio.2022.100351y Fulltext
856	4	u https://uu.diva-portal.org/smash/get/diva2:1683586/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-480694
856	4 8	u https://doi.org/10.1016/j.mtbio.2022.100351

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