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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004682naa a2200493 4500
001oai:gup.ub.gu.se/323938
003SwePub
008240528s2019 | |||||||||||000 ||eng|
0247 a https://gup.ub.gu.se/publication/3239382 URI
0247 a https://doi.org/10.3389/fphys.2019.006942 DOI
040 a (SwePub)gu
041 a eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Vega, Génesis4 aut
2451 0a Normal calcium-activated anion secretion in a mouse selectively lacking TMEM16A in intestinal epithelium
264 c 2019-06-13
264 1b Frontiers Media SA,c 2019
520 a Calcium-activated anion secretion is expected to ameliorate cystic fibrosis, a genetic disease that carries an anion secretory defect in exocrine tissues. Human patients and animal models of the disease that present a mild intestinal phenotype have been postulated to bear a compensatory calcium-activated anion secretion in the intestine. TMEM16A is calcium-activated anion channel whose presence in the intestinal epithelium is contradictory. We aim to test the functional expression of TMEM16A using animal models with Cftr and/or Tmem16a intestinal silencing. Expression of TMEM16A was studied in a wild type and intestinal Tmem16a knockout mice by mRNA-seq, mass-spectrometry, q-PCR, Western blotting and immunolocalization. Calcium-activated anion secretion was recorded in the ileum and proximal colon of these animals including intestinal Cftr knockout and double mutants with dual Tmem16a and Cftr intestinal ablation. Mucus homeostasis was studied by immune-analysis of Mucin-2 (Muc2) and survival curves were recorded. Tmem16a transcript was found in intestine. Nevertheless, protein was barely detected in colon samples. Electrophysiological measurements demonstrated that the intestinal deletion of Tmem16a did not change calcium-activated anion secretion induced by carbachol or ATP in ileum and proximal colon. Muc2 architecture was not altered by Tmem16a silencing as was observed when Cftr was deleted from mouse intestine. Tmem16a silencing neither affected animal survival nor modified the lethality observed in the intestinal Cftr-null mouse. Our results demonstrate that TMEM16A function in the murine intestine is not related to electrogenic calcium-activated anion transport and does not affect mucus homeostasis and survival of animals.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaper0 (SwePub)3012 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicine0 (SwePub)3012 hsv//eng
653 a Colon
653 a Cystic fibrosis transmembrane conductance regulator
653 a Epithelial transport
653 a Intestinal mucus
653 a TMEM16A
7001 a Guequén, Anita4 aut
7001 a Johansson, Malin E V,d 1971u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology4 aut0 (Swepub:gu)xjomal
7001 a Arike, Liisau Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology4 aut0 (Swepub:gu)xarili
7001 a Martinez Abad, Beatrizu Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology4 aut0 (Swepub:gu)xmartb
7001 a Nyström, Elisabeth E. L.u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine4 aut
7001 a Scudieri, Paolo4 aut
7001 a Pedemonte, Nicoletta4 aut
7001 a Millar-Büchner, Pamela4 aut
7001 a Philp, Amber R.4 aut
7001 a Galietta, Luis J.4 aut
7001 a Hansson, Gunnar C.,d 1951u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology4 aut0 (Swepub:gu)xhagun
7001 a Flores, Carlos A.4 aut
7102 a Göteborgs universitetb Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi4 org
7730 t Frontiers in Physiologyd : Frontiers Media SAg 10q 10x 1664-042X
8564 u https://www.frontiersin.org/articles/10.3389/fphys.2019.00694/pdf
8564 8u https://gup.ub.gu.se/publication/323938
8564 8u https://doi.org/10.3389/fphys.2019.00694

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