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Sökning: WFRF:(Li Qi 1990) > Improving recombina...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004477naa a2200457 4500
001oai:research.chalmers.se:03b036a9-eba7-4c6d-b6a9-76271f3225e4
003SwePub
008220616s2022 | |||||||||||000 ||eng|
0247 a https://research.chalmers.se/publication/5308302 URI
0247 a https://doi.org/10.1038/s41467-022-30689-72 DOI
040 a (SwePub)cth
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Li, Feiran,d 1993u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)feiranl
2451 0a Improving recombinant protein production by yeast through genome-scale modeling using proteome constraints
264 c 2022-05-27
264 1b Springer Science and Business Media LLC,c 2022
338 a electronic2 rdacarrier
520 a Eukaryotic cells are used as cell factories to produce and secrete multitudes of recombinant pharmaceutical proteins, including several of the current top-selling drugs. Due to the essential role and complexity of the secretory pathway, improvement for recombinant protein production through metabolic engineering has traditionally been relatively ad-hoc; and a more systematic approach is required to generate novel design principles. Here, we present the proteome-constrained genome-scale protein secretory model of yeast Saccharomyces cerevisiae (pcSecYeast), which enables us to simulate and explain phenotypes caused by limited secretory capacity. We further apply the pcSecYeast model to predict overexpression targets for the production of several recombinant proteins. We experimentally validate many of the predicted targets for alpha-amylase production to demonstrate pcSecYeast application as a computational tool in guiding yeast engineering and improving recombinant protein production. Due to the complexity of the protein secretory pathway, strategy suitable for the production of a certain recombination protein cannot be generalized. Here, the authors construct a proteome-constrained genome-scale protein secretory model for yeast and show its application in the production of different misfolded or recombinant proteins.
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
650 7a NATURVETENSKAPx Data- och informationsvetenskapx Bioinformatik0 (SwePub)102032 hsv//swe
650 7a NATURAL SCIENCESx Computer and Information Sciencesx Bioinformatics0 (SwePub)102032 hsv//eng
650 7a NATURVETENSKAPx Biologix Bioinformatik och systembiologi0 (SwePub)106102 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Bioinformatics and Systems Biology0 (SwePub)106102 hsv//eng
7001 a Chen, Yu,d 1990u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)cheyu
7001 a Qi, Qi,d 1992u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)fomde
7001 a Wang, Yanyan,d 1989u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)yanyanw
7001 a Yuan, Le,d 1994u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)leyu
7001 a Huang, Mingtao,d 1984u Chalmers tekniska högskola,Chalmers University of Technology,South China University of Technology4 aut0 (Swepub:cth)mingtao
7001 a El-Semman, Ibrahim,d 1977u Chalmers tekniska högskola,Chalmers University of Technology,Assiut University4 aut0 (Swepub:cth)elsemman
7001 a Feizi, Amir,d 1980u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)feizi
7001 a Kerkhoven, Eduard,d 1985u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)eduardk
7001 a Nielsen, Jens B,d 1962u Chalmers tekniska högskola,Chalmers University of Technology,BioInnovation Institute (BII)4 aut0 (Swepub:cth)nielsenj
7102 a Chalmers tekniska högskolab South China University of Technology4 org
7730 t Nature Communicationsd : Springer Science and Business Media LLCg 13:1q 13:1x 2041-1723x 2041-1723
8564 u https://research.chalmers.se/publication/530830/file/530830_Fulltext.pdfx primaryx freey FULLTEXT
8564 8u https://research.chalmers.se/publication/530830
8564 8u https://doi.org/10.1038/s41467-022-30689-7

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