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  • Brändén, Gisela,1975Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology (author)

Coherent diffractive imaging of microtubules using an X-ray laser.

  • Article/chapterEnglish2019

Publisher, publication year, extent ...

  • 2019-06-13
  • Springer Science and Business Media LLC,2019

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/281377
  • https://gup.ub.gu.se/publication/281377URI
  • https://doi.org/10.1038/s41467-019-10448-xDOI

Supplementary language notes

  • Language:English

Part of subdatabase

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate approach: the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Hammarin, Greger,1981Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xhamgr (author)
  • Harimoorthy, RajivGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xharra (author)
  • Johansson, AlexanderGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology (author)
  • Arnlund, DavidGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xarnda (author)
  • Malmerberg, Erik (author)
  • Barty, Anton (author)
  • Tångefjord, Stefan (author)
  • Berntsen, Peter,1974Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology (author)
  • DePonte, Daniel P (author)
  • Seuring, Carolin (author)
  • White, Thomas A (author)
  • Stellato, Francesco (author)
  • Bean, Richard (author)
  • Beyerlein, Kenneth R (author)
  • Chavas, Leonard M G (author)
  • Fleckenstein, Holger (author)
  • Gati, Cornelius (author)
  • Ghoshdastider, Umesh (author)
  • Gumprecht, Lars (author)
  • Oberthür, Dominik (author)
  • Popp, David (author)
  • Seibert, Marvin (author)
  • Tilp, Thomas (author)
  • Messerschmidt, Marc (author)
  • Williams, Garth J (author)
  • Loh, N Duane (author)
  • Chapman, Henry N (author)
  • Zwart, Peter (author)
  • Liang, Mengning (author)
  • Boutet, Sébastien (author)
  • Robinson, Robert C (author)
  • Neutze, Richard,1969Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xneuri (author)
  • Göteborgs universitetInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

Related titles

  • In:Nature communications: Springer Science and Business Media LLC10:12041-1723

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