Multiple genotypic aberrances associate to terminal differentiation-deficiency of an oral squamous cell carcinoma in serum-free culture

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: WFRF:(Grafström Roland) > Multiple genotypic ...

LIBRIS Formathandbok (Information om MARC21)

Fältnamn	Indikatorer	Metadata
000		03723naa a2200361 4500
001		oai:DiVA.org:liu-43445
003		SwePub
008		091010s2008 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:prod.swepub.kib.ki.se:117637630
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-434452 URI
024	7	a https://doi.org/10.1111/j.1432-0436.2008.00267.x2 DOI
024	7	a http://kipublications.ki.se/Default.aspx?queryparsed=id:1176376302 URI
040		a (SwePub)liud (SwePub)ki
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Roberg, Karin,d 1957-u Linköpings universitet,Oto-Rhino-Laryngologi,Hälsouniversitetet4 aut0 (Swepub:liu)karro26
245	1 0	a Multiple genotypic aberrances associate to terminal differentiation-deficiency of an oral squamous cell carcinoma in serum-free culture
264	1	b Elsevier BV,c 2008
338		a print2 rdacarrier
520		a Oral squamous cell carcinoma (OSCC) lines proliferative in the serum-free conditions devised for normal oral keratinocytes (NOK) are virtually absent, complicating studies of carcinogenesis. A tongue squamous cell carcinoma generated under conditions for normal cell culture an apparently immortal line (termed LK0412) that has undergone ≥200 population doublings from over a year in culture. LK0412 exhibited epithelial morphology, intermediate filaments, desmosomes, and cytokeratin. Soft agar growth and tumorigenicity in athymic nude mice indicated the malignant phenotype. Compared with NOK, LK0412 exhibited increased indices for proliferation and apoptosis, and a decreased terminal differentiation index. Fetal bovine serum inhibited growth and increased apoptosis but failed to induce terminal differentiation of LK0412; the latter outcome differed clearly from that in NOK. Gene ontology assessment of transcript profiles implicated multiple alterations in biological processes, molecular functions, and cellular components in LK0412. Genetic changes, some that were confirmed to the protein level, included previously proposed OSCC markers, i.e., BAX, CDC2, and TP53, as well as multiple cancer-associated genes not considered for OSCC, e.g., BST2, CRIP1, ISG15, KLRC1, NEDD9, NNMT, and TWIST1. Elevation of p53 protein agreed with a missense mutation detectable in both the LK0412 line and the original tumor specimen. Moderate differentiation characterized the original tumor as well as tumors generated from inoculation of LK0412 in mice. Overall, the results suggest that the LK0412 cell line represent a subgroup of OSCC with unique genomic and phenotypic profiles. LK0412 should be useful to exploration of OSCC development, particularly the deregulated growth and differentiation responsiveness to serum factors.
653		a MEDICINE
653		a MEDICIN
700	1	a Ceder, Rebeccau Karolinska Institutet4 aut
700	1	a Farnebo, Lovisa,d 1973-u Linköpings universitet,Oto-Rhino-Laryngologi,Hälsouniversitetet4 aut0 (Swepub:liu)lovfa06
700	1	a Norberg-Spaak, Lena,d 1956-u Linköpings universitet,Oto-Rhino-Laryngologi,Hälsouniversitetet4 aut0 (Swepub:liu)lenno72
700	1	a Grafström, Rolandu Division of Biochemical Toxicology and Experimental Cancer Research, Institute of Environmental Medicine, Karolinska Institute4 aut
710	2	a Linköpings universitetb Oto-Rhino-Laryngologi4 org
773	0	t Differentiationd : Elsevier BVg 76:8, s. 868-880q 76:8<868-880x 0301-4681x 1432-0436
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-43445
856	4 8	u https://doi.org/10.1111/j.1432-0436.2008.00267.x
856	4 8	u http://kipublications.ki.se/Default.aspx?queryparsed=id:117637630

Hitta via bibliotek

Differentiation (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

Hitta mer i SwePub

Av författaren/redakt...: Roberg, Karin, 1 ...; Ceder, Rebecca; Farnebo, Lovisa, ...; Norberg-Spaak, L ...; Grafström, Rolan ...

Artiklar i publikationen: Differentiation

Av lärosätet: Linköpings universitet; Karolinska Institutet

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se