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  • Kim, Jung HunDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea (författare)

Production of β-carotene by recombinant Escherichia coli with engineered whole mevalonate pathway in batch and fed-batch cultures

  • Artikel/kapitelEngelska2009

Förlag, utgivningsår, omfång ...

  • 2009-11-05
  • Springer Science and Business Media LLC,2009
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:kth-297825
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-297825URI
  • https://doi.org/10.1007/s12257-008-0230-1DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • QC 20210824
  • Recombinant Escherichia coli engineered to contain the whole mevalonate pathway and foreign genes for β-carotene biosynthesis, was utilized for production of β-carotene in bioreactor cultures. Optimum culture conditions were established in batch and pH-stat fed-batch cultures to determine the optimal feeding strategy thereby improving production yield. The specific growth rate and volumetric productivity in batch cultures at 37°C were 1.7-fold and 2-fold higher, respectively, than those at 28°C. Glycerol was superior to glucose as a carbon source. Maximum β-carotene production (titer of 663 mg/L and overall volumetric productivity of 24.6 mg/L × h) resulted from the simultaneous addition of 500 g/L glycerol and 50 g/L yeast extract in pH-stat fed-batch culture.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Kim, Seon WonDivision of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea (författare)
  • Nguyen, Do Quynh AnhDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea (författare)
  • Li, He,D.Sc,1985-Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea(Swepub:kth)u192ayff (författare)
  • Kim, Sung BaeDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea (författare)
  • Seo, Yang GonDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea (författare)
  • Yang, Jae KyungDivision of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, 660-701, Korea (författare)
  • Chung, In YoungDepartment of Electronic and Communications Engineering, Kwangwoon University, Seoul, 139-701, Korea (författare)
  • Kim, Dae HwanSchool of Electrical Engineering, Kookmin University, Seoul, 136-702, Korea (författare)
  • Kim, Chang JoonDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea (författare)
  • Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, KoreaDivision of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Biotechnology and Bioprocess Engineering: Springer Science and Business Media LLC14:5, s. 559-5641226-83721976-3816

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