Sökning: WFRF:(Froese B) > Ancient bacteria sh...
Fältnamn | Indikatorer | Metadata |
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000 | 03553naa a2200505 4500 | |
001 | oai:lup.lub.lu.se:c6b2cb42-0c38-479c-8f2e-079460e66d4d | |
003 | SwePub | |
008 | 160401s2007 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/6569652 URI |
024 | 7 | a https://doi.org/10.1073/pnas.07067871042 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Johnson, Sarah Stewart4 aut |
245 | 1 0 | a Ancient bacteria show evidence of DNA repair |
264 | c 2007-09-04 | |
264 | 1 | b Proceedings of the National Academy of Sciences,c 2007 |
520 | a Recent claims of cultivable ancient bacteria within sealed environments highlight our limited understanding of the mechanisms behind long-term cell survival. It remains unclear how dormancy, a favored explanation for extended cellular persistence, can cope with spontaneous genomic decay over geological timescales. There has been no direct evidence in ancient microbes for the most likely mechanism, active DNA repair, or for the metabolic activity necessary to sustain it. In this paper, we couple PCR and enzymatic treatment of DNA with direct respiration measurements to investigate long-term survival of bacteria sealed in frozen conditions for up to one million years. Our results show evidence of bacterial survival in samples up to half a million years in age, making this the oldest independently authenticated DNA to date obtained from viable cells. Additionally, we find strong evidence that this long-term survival is closely tied to cellular metabolic activity and DNA repair that over time proves to be superior to dormancy as a mechanism in sustaining bacteria viability. | |
650 | 7 | a NATURVETENSKAPx Geovetenskap och miljövetenskapx Naturgeografi0 (SwePub)105072 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Earth and Related Environmental Sciencesx Physical Geography0 (SwePub)105072 hsv//eng |
653 | a DNA damage | |
653 | a long-term microbial survival | |
653 | a metabolic activity | |
700 | 1 | a Hebsgaard, Martin B.4 aut |
700 | 1 | a Christensen, Torbenu Lund University,Lunds universitet,Institutionen för naturgeografi och ekosystemvetenskap,Naturvetenskapliga fakulteten,Dept of Physical Geography and Ecosystem Science,Faculty of Science4 aut0 (Swepub:lu)ekol-tch |
700 | 1 | a Mastepanov, Mikhailu Lund University,Lunds universitet,Institutionen för naturgeografi och ekosystemvetenskap,Naturvetenskapliga fakulteten,Dept of Physical Geography and Ecosystem Science,Faculty of Science4 aut0 (Swepub:lu)ple-mma |
700 | 1 | a Nielsen, Rasmus4 aut |
700 | 1 | a Munch, Kasper4 aut |
700 | 1 | a Brand, Tina4 aut |
700 | 1 | a Thomas, M.4 aut |
700 | 1 | a Gilbert, P.4 aut |
700 | 1 | a Zuber, Maria T.4 aut |
700 | 1 | a Bunce, Michael4 aut |
700 | 1 | a Ronn, Regin4 aut |
700 | 1 | a Gilichinsky, David4 aut |
700 | 1 | a Froese, Duane4 aut |
700 | 1 | a Willerslev, Eske4 aut |
710 | 2 | a Institutionen för naturgeografi och ekosystemvetenskapb Naturvetenskapliga fakulteten4 org |
773 | 0 | t Proceedings of the National Academy of Sciencesd : Proceedings of the National Academy of Sciencesg 104:36, s. 14401-14405q 104:36<14401-14405x 1091-6490x 0027-8424 |
856 | 4 | u http://dx.doi.org/10.1073/pnas.0706787104x freey FULLTEXT |
856 | 4 | u https://europepmc.org/articles/pmc1958816?pdf=render |
856 | 4 8 | u https://lup.lub.lu.se/record/656965 |
856 | 4 8 | u https://doi.org/10.1073/pnas.0706787104 |
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