YscU/FlhB of Yersinia pseudotuberculosis Harbors a C-terminal Type III Secretion Signal

Login, Frederic H. (författare): Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Umeå Centre for Microbial Research (UCMR)

Wolf-Watz, Hans (författare): Umeå universitet,Molekylär Infektionsmedicin, Sverige (MIMS),Umeå Centre for Microbial Research (UCMR)

(creator_code:org_t)

Umea Univ, Dept Mol Biol, Umea Ctr Microbial Res, SE-90187 Umea, Sweden. Umea Univ, Lab Mol Infect Med Sweden MIMS, Umea Ctr Microbial Res, SE-90187 Umea, Sweden. 2015
2015
Engelska.
Ingår i: Journal of Biological Chemistry. - Umea Univ, Dept Mol Biol, Umea Ctr Microbial Res, SE-90187 Umea, Sweden. Umea Univ, Lab Mol Infect Med Sweden MIMS, Umea Ctr Microbial Res, SE-90187 Umea, Sweden.. - 0021-9258 .- 1083-351X. ; 290:43, s. 26282-26291

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Abstract Ämnesord

Stäng

All type III secretion systems (T3SS) harbor a member of the YscU/FlhB family of proteins that is characterized by an auto-proteolytic process that occurs at a conserved cytoplasmic NPTH motif. We have previously demonstrated that YscU(CC), the C-terminal peptide generated by auto-proteolysis of Yersinia pseudotuberculosis YscU, is secreted by the T3SS when bacteria are grown in Ca2+ -depleted medium at 37 degrees C. Here, we investigated the secretion of this early T3S-substrate and showed that YscU(CC) encompasses a specific C-terminal T3S signal within the 15 last residues (U-15). U-15 promoted C-terminal secretion of reporter proteins like GST and YopE lacking its native secretion signal. Similar to the "classical" N-terminal secretion signal, U-15 interacted with the ATPase YscN. Although U-15 is critical for YscU(CC) secretion, deletion of the C-terminal secretion signal of YscU(CC) did neither affect Yop secretion nor Yop translocation. However, these deletions resulted in increased secretion of YscF, the needle subunit. Thus, these results suggest that YscU via its C-terminal secretion signal is involved in regulation of the YscF secretion.

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