Isolation of buffalo poxvirus from clinical case and variations in the genetics of the B5R gene over fifty passages

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Fältnamn	Indikatorer	Metadata
000		03029naa a2200421 4500
001		oai:DiVA.org:uu-260827
003		SwePub
008		150825s2015 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2608272 URI
024	7	a https://doi.org/10.1007/s11262-015-1209-62 DOI
040		a (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Goraya, Mohsan Ullahu Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi4 aut
245	1 0	a Isolation of buffalo poxvirus from clinical case and variations in the genetics of the B5R gene over fifty passages
264		c 2015-06-13
264	1	b Springer Science and Business Media LLC,c 2015
338		a print2 rdacarrier
520		a Outbreaks of buffalopox affect udder and teats, which may ultimately lead to mastitis in dairy buffalo and can significantly compromise the production. In this study, we report isolation of buffalo poxvirus and sequence analysis of the B5R gene collected from the buffalo clinically suspected to be poxvirus infected. The virus was isolated on BHK-21 cell line and was passaged for 50 times, B5R gene was amplified and sequenced using gene-specific primers, and analyzed at both nucleotide and amino acid levels. Phylogenetically, the isolate can be classified close to the previously reported Pakistani and Indian isolates with certain level of differential clustering patterns. Three significant putative mutations (I2K, N64D, and K111E) were observed in the B5R protein. The K111E was common with previous human isolate from Karachi, Pakistan in 2005. These mutations differed from pox-viruses reported from the neighboring countries. Some deletion mutations were observed which were recovered in upcoming passages. The K111E mutation suggests potential to cause zoonotic infection in human all over the country.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Infektionsmedicin0 (SwePub)302092 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Infectious Medicine0 (SwePub)302092 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Medicinsk genetik0 (SwePub)301072 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Medical Genetics0 (SwePub)301072 hsv//eng
653		a Buffalo poxvirus
653		a B5Rgene
653		a Zoonosis
653		a Host range
653		a Phylogeny
700	1	a Qureshi, Zafar ul Ahsan4 aut
700	1	a Abbas, Muhammad4 aut
700	1	a Ashraf, Muhammad4 aut
700	1	a Munir, Muhammad4 aut
710	2	a Uppsala universitetb Institutionen för medicinsk biokemi och mikrobiologi4 org
773	0	t Virus genesd : Springer Science and Business Media LLCg 51:1, s. 45-50q 51:1<45-50x 0920-8569x 1572-994X
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-260827
856	4 8	u https://doi.org/10.1007/s11262-015-1209-6

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Infektionsmedici ...

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