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Engineering the car...
Engineering the carotenoid biosynthetic pathway in Rhodothermus marinus for lycopene production
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- Kristjansdottir, Thordis (författare)
- Matís ltd. – Icelandic food and biotech R&D,University of Iceland
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- Ron, Emanuel Y.C. (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Molins-Delgado, Daniel (författare)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Fridjonsson, Olafur H. (författare)
- Matís ltd. – Icelandic food and biotech R&D
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- Turner, Charlotta (författare)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Bjornsdottir, Snaedis H. (författare)
- University of Iceland
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- Gudmundsson, Steinn (författare)
- University of Iceland
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- van Niel, Ed W.J. (författare)
- Lund University,Lunds universitet,Teknisk mikrobiologi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Applied Microbiology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Karlsson, Eva Nordberg (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Hreggvidsson, Gudmundur O. (författare)
- University of Iceland,Matís ltd. – Icelandic food and biotech R&D
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Matís ltd – Icelandic food and biotech R&D University of Iceland (creator_code:org_t)
- Elsevier BV, 2020
- 2020
- Engelska.
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Ingår i: Metabolic Engineering Communications. - : Elsevier BV. - 2214-0301. ; 11
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Rhodothermus marinus has the potential to be well suited for biorefineries, as an aerobic thermophile that produces thermostable enzymes and is able to utilize polysaccharides from different 2nd and 3rd generation biomass. The bacterium produces valuable chemicals such as carotenoids. However, the native carotenoids are not established for industrial production and R. marinus needs to be genetically modified to produce higher value carotenoids. Here we genetically modified the carotenoid biosynthetic gene cluster resulting in three different mutants, most importantly the lycopene producing mutant TK-3 (ΔtrpBΔpurAΔcruFcrtB::trpBcrtBT.thermophilus). The genetic modifications and subsequent structural analysis of carotenoids helped clarify the carotenoid biosynthetic pathway in R. marinus. The nucleotide sequences encoding the enzymes phytoene synthase (CrtB) and the previously unidentified 1′,2′-hydratase (CruF) were found fused together and encoded by a single gene in R. marinus. Deleting only the cruF part of the gene did not result in an active CrtB enzyme. However, by deleting the entire gene and inserting the crtB gene from Thermus thermophilus, a mutant strain was obtained, producing lycopene as the sole carotenoid. The lycopene produced by TK-3 was quantified as 0.49 g/kg CDW (cell dry weight).
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
Nyckelord
- Carotenoids
- Genetic engineering
- Lycopene
- Rhodothermus marinus
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Kristjansdottir, ...
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Ron, Emanuel Y.C ...
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Molins-Delgado, ...
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Fridjonsson, Ola ...
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Turner, Charlott ...
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Bjornsdottir, Sn ...
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Gudmundsson, Ste ...
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van Niel, Ed W.J ...
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Karlsson, Eva No ...
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Hreggvidsson, Gu ...
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Lunds universitet