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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004642naa a2200505 4500
001oai:DiVA.org:kth-147031
003SwePub
008140623s2014 | |||||||||||000 ||eng|
009oai:DiVA.org:uu-227710
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-1470312 URI
0247 a https://doi.org/10.1093/molehr/gau0182 DOI
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2277102 URI
040 a (SwePub)kthd (SwePub)uu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Djureinovic, Dijanau Uppsala universitet,Molekylär och morfologisk patologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:uu)dijdj435
2451 0a The human testis-specific proteome defined by transcriptomics and antibody-based profiling
264 c 2014-03-05
264 1b Oxford University Press (OUP),c 2014
338 a print2 rdacarrier
500 a QC 20140624
520 a The testis' function is to produce haploid germ cells necessary for reproduction. Here we have combined a genome-wide transcriptomics analysis with immunohistochemistry-based protein profiling to characterize the molecular components of the testis. Deep sequencing (RNA-Seq) of normal human testicular tissue from seven individuals was performed and compared with 26 other normal human tissue types. All 20 050 putative human genes were classified into categories based on expression patterns. The analysis shows that testis is the tissue with the most tissue-specific genes by far. More than 1000 genes show a testis-enriched expression pattern in testis when compared with all other analyzed tissues. Highly testis enriched genes were further characterized with respect to protein localization within the testis, such as spermatogonia, spermatocytes, spermatids, sperm, Sertoli cells and Leydig cells. Here we present an immunohistochemistry-based analysis, showing the localization of corresponding proteins in different cell types and various stages of spermatogenesis, for 62 genes expressed at > 50-fold higher levels in testis when compared with other tissues. A large fraction of these genes were unexpectedly expressed in early stages of spermatogenesis. In conclusion, we have applied a genome-wide analysis to identify the human testis-specific proteome using transcriptomics and antibody-based protein profiling, providing lists of genes expressed in a tissue-enriched manner in the testis. The majority of these genes and proteins were previously poorly characterised in terms of localization and function, and our list provides an important starting point to increase our molecular understanding of human reproductive biology and disease.
650 7a NATURVETENSKAPx Biologix Utvecklingsbiologi0 (SwePub)106142 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Developmental Biology0 (SwePub)106142 hsv//eng
650 7a NATURVETENSKAPx Biologix Evolutionsbiologi0 (SwePub)106152 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Evolutionary Biology0 (SwePub)106152 hsv//eng
653 a immunohistochemistry
653 a RNA sequencing
653 a spermatogenesis
653 a testis
653 a tissue specificity
7001 a Fagerberg, Linnu KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1dglfid
7001 a Hallström, Björnu KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1ih19oh
7001 a Danielsson, A.u Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab4 aut
7001 a Lindskog Bergström, Ceciliau Uppsala universitet,Molekylär och morfologisk patologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:uu)cecli129
7001 a Uhlén, Mathiasu KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1dulvmw
7001 a Pontén, Fredriku Uppsala universitet,Molekylär och morfologisk patologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:uu)fredpont
7102 a Uppsala universitetb Molekylär och morfologisk patologi4 org
7730 t Molecular human reproductiond : Oxford University Press (OUP)g 20:6, s. 476-488q 20:6<476-488x 1360-9947x 1460-2407
8564 u https://academic.oup.com/molehr/article-pdf/20/6/476/13817047/gau018.pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-147031
8564 8u https://doi.org/10.1093/molehr/gau018
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-227710

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