Development of a fed-batch process for the production of a recombinant protein X in CHO-GS system : Case study from the cell to reactor process ready for pilot scale cultivation

• A new cell line was created using CHO-GS system. The most promising clones were adapted to different base cultivation media leading to the selection of one medium. The fed-batch process development was performed in spinner, shake flask and bioreactor scale. It included the selection of a feed medium, the choice of the feed strategy and the optimisation of the glucose feeding. The process was then simplified by using a single feed including the feed medium and the glucose feed. Finally up-scaling parameters like aeration and CO2 stripping were studied in 3 L and 15 L bioreactors in preparation for pilot scale operation. This process proved to be robust, reproducible and suitable for large and commercial scale operation.

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik -- Läkemedelsbioteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology -- Pharmaceutical Biotechnology (hsv//eng)

Nyckelord

CHO·GS·Fed-batch·Feed·Up-scale·Bioreactors

SRA - Molecular Bioscience

SRA - Molekylär biovetenskap

Publikations- och innehållstyp

vet (ämneskategori)

kon (ämneskategori)