A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers

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Fältnamn	Indikatorer	Metadata
000		02808naa a2200349 4500
001		oai:DiVA.org:uu-259346
003		SwePub
008		150731s2015 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2593462 URI
024	7	a https://doi.org/10.1186/s12950-015-0090-32 DOI
040		a (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Nilsen, Tomu Uppsala universitet,Biokemisk struktur och funktion4 aut0 (Swepub:uu)tomni279
245	1 0	a A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers
264		c 2015-07-25
264	1	b Springer Science and Business Media LLC,c 2015
338		a electronic2 rdacarrier
500		a Economical support was given from the Norwegian Research Council.
520		a Serum and plasma calprotectin concentration is shown to be elevated when neutrophils are activated, and may therefore be used as a marker for inflammatory diseases. A serum calprotectin immunoassay was developed based on calprotectin values observed in samples from the intensive care unit. The polyclonal avian antibodies were raised and affinity purified with calprotectin antigens. The performance was tested and it was observed that the assay was linear in the range 0.3-24.7 mg/L, the limit of quantitation was observed to be lower than 0.3 mg/L, no antigen excess was observed up to 54 mg/L, all CVs were lower than 1.8 % in the precision study, the calibration curve stability was longer than 6 weeks, and there was no significant interference detected for haemoglobin, intralipid or bilirubin. The serum calprotectin immunoassay presented in this paper performs well within the criteria carefully set from the limited clinical experience obtained in both serum and plasma. In addition it is commutable with Bühlmann MRP8/14 ELISA.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Infektionsmedicin0 (SwePub)302092 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Infectious Medicine0 (SwePub)302092 hsv//eng
700	1	a Sunde, Kathrin4 aut
700	1	a Larsson, Andersu Uppsala universitet,Biokemisk struktur och funktion4 aut0 (Swepub:uu)andlarss
710	2	a Uppsala universitetb Biokemisk struktur och funktion4 org
773	0	t Journal of Inflammationd : Springer Science and Business Media LLCg 12q 12x 1476-9255
856	4	u https://uu.diva-portal.org/smash/get/diva2:843856/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
856	4	u https://journal-inflammation.biomedcentral.com/track/pdf/10.1186/s12950-015-0090-3
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-259346
856	4 8	u https://doi.org/10.1186/s12950-015-0090-3

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Av författaren/redakt...: Nilsen, Tom; Sunde, Kathrin; Larsson, Anders

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Infektionsmedici ...

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