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WFRF:(Holmqvist Bo)
 

Sökning: WFRF:(Holmqvist Bo) > THE INSULIN-LIKE GR...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004893naa a2200313 4500
001oai:lup.lub.lu.se:1b78b245-e0f7-458d-b863-4e4b33d9a410
003SwePub
008190925s2019 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/1b78b245-e0f7-458d-b863-4e4b33d9a4102 URI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a kon2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Ekström, Claesu Lund University,Lunds universitet,Neonatalogi,Forskargrupper vid Lunds universitet,Sjukdomsorsakande oxidation,Neonatology,Lund University Research Groups,Redox Medicine4 aut0 (Swepub:lu)cl0514ek
2451 0a THE INSULIN-LIKE GROWTH FACTOR 1 (IGF-1) SYSTEM IN THE PRETERM RABBIT PUP : A CHARACTERIZATION OF THE IGF-1 MRNA EXPRESSION IN LIVER, IGF-1 PROTEIN LEVELS IN SERUM AND BRAIN DISTRIBUTION OF IGF-1 RECEPTORS
264 1c 2019
520 a IGF-1 is an essential regulator of fetal growth and brain development. Preterm birth in the human is followed by a rapid decrease in serum levels of IGF-1 and decreased levels of IGF-1 have been associated with development of severemorbidity. A recent clinical trial indicated that supplementation with IGF-1 prevented development of severe intraventricular hemorrhage (IVH) in extremely preterm infants. In order to better understand possible mechanisms involved in IGF-1-induced IVH prevention, we evaluated important aspects of the endogenous IGF-1 system; IGF-I mRNA expression in the liver and associated serum protein levels and brain IGF-1 receptor (IGFR) distribution in the preterm rabbit.Rabbit pups were delivered by cesarean section at E29 (preterm) or by vaginal delivery (term = E32), housed in a controlled environment and fed twice daily with bovine colostrum via a gastric tube. Serum concentrations of IGF-1protein and liver expression of IGF-1 mRNA were determined at 0, 2, 6, 12, 24, 48 and 72 h of age in preterm pups. Paraffin brain sections from perfusion fixed untreated animals (preterm pups at 20 h and term pups at 5-7 h and 96 h) were prepared for immunohistochemistry against IGF1R, by labeling with primary antibodies against IGF1R, and processed for chromogen visualization and density/quantitation analysis with confocal microscopy. Mean (SD) serum concentrations of IGF-1 decreased from 166 (33) ng/ml at birth (E29) to 28 (9) ng/ml at day 3 (P0). Hepatic expression of IGF-1 mRNA did not vary over time. The IGF1R was widely distributed in multiple brain regions in both preterm and term pups (Fig). The most abundant density of IGF1R was observed in the choroid plexus, the subfornical organ, the meninges, major fiber tracts, the cortex and sub-ependymal germinal zones. The IGF1R, was mainly localized on outer cell membranes, on cell bodies and along nerve fibers. Quantitative analysis of IGF1R immunoreactivity showed similar IGF1R densities in preterm and term pups of corresponding ages. IGF1R density decreased with increasing postnatal age in term pups.In line with what is observed in the preterm human infant, serum protein levels of IGF-1 in the preterm rabbit pup decrease rapidly following birth. The IGF1R is widely expressed in the brain following birth, with high expressions in regions and structures relevant for vessel rupture in IVH. The preterm rabbit thus presents a well-suited model for characterization and evaluation of mechanisms involved in IGF-1 induced prevention of IVH.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Farmakologi och toxikologi0 (SwePub)301022 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Pharmacology and Toxicology0 (SwePub)301022 hsv//eng
7001 a Holmqvist, Bo4 aut
7001 a Gram, Magnusu Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Antioxidationsmedicin,Forskargrupper vid Lunds universitet,Neonatalogi,Sjukdomsorsakande oxidation,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Antioxidation medicine,Lund University Research Groups,Neonatology,Redox Medicine4 aut0 (Swepub:lu)medk-mo0
7001 a Ley, Davidu Lund University,Lunds universitet,Pediatrik, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Neonatalogi,Forskargrupper vid Lunds universitet,Paediatrics (Lund),Section V,Department of Clinical Sciences, Lund,Faculty of Medicine,Neonatology,Lund University Research Groups4 aut0 (Swepub:lu)pedi-dle
7001 a Carey, Galen4 edt
7001 a Wang, Xiaoyang4 edt
7001 a Hellström, Ann4 edt
7001 a Barton, Norman W.u Takeda Pharmaceuticals International4 edt
7102 a Neonatalogib Forskargrupper vid Lunds universitet4 org
8564 8u https://lup.lub.lu.se/record/1b78b245-e0f7-458d-b863-4e4b33d9a410

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