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Monitoring of autop...
Monitoring of autophagy is complicated : Salinomycin as an example
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- Reddy Jangamreddy, Jaganmohan, 1977- (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
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- Panigrahi, Soumya (författare)
- Department of Medicine/Infectious Diseases, Case Western Reserve University, Cleveland, USA
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- Los, Marek J. (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet,Pomeranian Medical University, Poland
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(creator_code:org_t)
- Elsevier, 2015
- 2015
- Engelska.
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Ingår i: Biochimica et Biophysica Acta. Molecular Cell Research. - : Elsevier. - 0167-4889 .- 1879-2596. ; 1853:3, s. 604-610
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Monitoring of autophagy is challenging because of its multiple steps and lack of single befitting technique for a complete mechanistic understanding, which makes the task complicated. Here, we evaluate the functionality of autophagy triggered by salinomycin (anti-cancer stem cell agent) using flow cytometry and advanced microscopy. We show that salinomycin does induce functional autophagy at lower concentrations and such a dose is cell type-dependent. For example, PC3 cells show active autophagic flux at 10μM concentration of salinomycin while murine embryonic fibroblasts already show an inhibition of flux at such doses. A higher concentration of salinomycin (i.e. 30μM) inhibits autophagic flux in both cell types. The data confirms our previous findings that salinomycin is an inducer of autophagy, whereas autophagic flux inhibition is a secondary response.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Autophagic flux; GFP-LC3; Salinomycin; Vacuolization; mTandem GFP-RFP LC3; p62/SQSTRM1
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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