Fast and Sensitive Method for the Determination of 17 Designer Benzodiazepines in Hair by Liquid Chromatography-Tandem Mass Spectrometry

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Fältnamn	Indikatorer	Metadata
000		03808naa a2200349 4500
001		oai:DiVA.org:liu-187516
003		SwePub
008		220825s2022 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1875162 URI
024	7	a https://doi.org/10.1093/jat/bkac0442 DOI
040		a (SwePub)liu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a DeFreitas, Laurau CUNY, NY 10019 USA; Columbia Univ, NY 10032 USA4 aut
245	1 0	a Fast and Sensitive Method for the Determination of 17 Designer Benzodiazepines in Hair by Liquid Chromatography-Tandem Mass Spectrometry
264		c 2022-06-24
264	1	b OXFORD UNIV PRESS INC,c 2022
338		a print2 rdacarrier
520		a In recent years, identification and analysis of designer benzodiazepines have become a challenge in forensic toxicology. These substances are analogs of the classic benzodiazepines, but their pharmacology is not well known, and many of them have been associated with overdoses and deaths. As a result, there has been a surge in efforts to develop analytical methods to determine these compounds in different biological samples. Our aim was to develop and validate a fast, sensitive and specific method for determining 17 designer benzodiazepines (adinazolam, clobazam, clonazolam, delorazepam, deschloroetizolam, diclazepam, etizolam, flualprazolam, flubromazepam, flubromazolam, flunitrazolam, N-desmethylclobazam, nifoxipam, nitrazolam, meclonazepam, pyrazolam and zolazepam) in hair by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Hair samples were decontaminated and pulverized, and a 20 mg aliquot was incubated in methanol in an ultrasound bath (1 h, 25 degrees C). The supernatant was evaporated and reconstituted in 200 mu L of mobile phase, and the extracts were filtered (nano-filter vials) before injection into LC-MS-MS. All analytes were eluted from the chromatographic column in 8 min, and two multiple-reaction monitoring (MRM) transitions were used to identify each compound. The limits of quantification were 5 or 25 pg/mg depending on the analyte, and the calibration functions were linear to 200 pg/mg. Imprecision was <19.2% (n = 15), and bias was from -13.7 to 18.3% (n = 15). All the analytes yielded high extraction efficiencies >70% and displayed ion suppression between -62.8% and -23.9% (n = 10). The method was applied to 19 authentic cases. Five samples were positive for flualprazolam ( 200 pg/mg) and/or etizolam (47.4-88.5 pg/mg). In conclusion, the present validated method has proven to be fast, sensitive, specific and capable of determining 17 designer benzodiazepines in hair using LC-MS-MS.
650	7	a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
700	1	a Fonseca Pego, Ana Miguelu CUNY, NY 10019 USA4 aut
700	1	a Kronstrand, Robert,d 1966-u Linköpings universitet,Avdelningen för klinisk kemi och farmakologi,Medicinska fakulteten,Natl Board Forens Med, Dept Forens Genet & Forens Toxicol, S-58758 Linkoping, Sweden4 aut0 (Swepub:liu)robkr41
700	1	a Lendoiro, Elenau Univ Santiago de Compostela, Spain4 aut
700	1	a De Castro-Rios, Anau Univ Santiago de Compostela, Spain4 aut
700	1	a Concheiro, Martau CUNY, NY 10019 USA4 aut
710	2	a CUNY, NY 10019 USA; Columbia Univ, NY 10032 USAb CUNY, NY 10019 USA4 org
773	0	t Journal of Analytical Toxicologyd : OXFORD UNIV PRESS INCg 46:8, s. 852-859q 46:8<852-859x 0146-4760x 1945-2403
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-187516
856	4 8	u https://doi.org/10.1093/jat/bkac044

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Av författaren/redakt...: DeFreitas, Laura; Fonseca Pego, An ...; Kronstrand, Robe ...; Lendoiro, Elena; De Castro-Rios, ...; Concheiro, Marta

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