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Artificial Polymeri...
Artificial Polymeric Scaffolds as Extracellular Matrix Substitutes for Autologous Conjunctival Goblet Cell Expansion
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- He, Min (författare)
- Harvard Medical Sch, MA USA; Shanxi Medical University, Peoples R China
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- Storr-Paulsen, Thomas (författare)
- Harvard Medical Sch, MA USA; Aarhus University Hospital NBG, Denmark
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- Wang, Annie L. (författare)
- Harvard Medical Sch, MA USA
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- Ghezzi, Chiara E. (författare)
- Tufts University, MA 02155 USA
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- Wang, Siran (författare)
- Tufts University, MA 02155 USA
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- Fullana, Matthew (författare)
- Case Western Reserve University, OH 44106 USA
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- Karamichos, Dimitrios (författare)
- University of Oklahoma, OK USA
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- Utheim, Tor P. (författare)
- Harvard Medical Sch, MA USA; University of Oslo, Norway; Vestre Viken Hospital Trust, Norway; University of Coll Southeast Norway, Norway
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- Islam, Rakibul (författare)
- Harvard Medical Sch, MA USA; University of Oslo, Norway
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- Griffith, May (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten,Karolinska Institute, Sweden
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- Islam, Mohammad Mirazul Mirazul (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten,Karolinska Institute, Sweden
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- Hodges, Robin R. (författare)
- Harvard Medical Sch, MA USA
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- Wnek, Gary E. (författare)
- Case Western Reserve University, OH 44106 USA
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- Kaplan, David L. (författare)
- Tufts University, MA 02155 USA
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- Dartt, Darlene A. (författare)
- Harvard Medical Sch, MA USA
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(creator_code:org_t)
- ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2016
- 2016
- Engelska.
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Ingår i: Investigative Ophthalmology and Visual Science. - : ASSOC RESEARCH VISION OPHTHALMOLOGY INC. - 0146-0404 .- 1552-5783. ; 57:14, s. 6134-6146
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- PURPOSE. We fabricated and investigated polymeric scaffolds that can substitute for the conjunctival extracellular matrix to provide a substrate for autologous expansion of human conjunctival goblet cells in culture. METHODS. We fabricated two hydrogels and two silk films: (1) recombinant human collagen (RHC) hydrogel, (2) recombinant human collagen 2-methacryloylxyethyl phosphorylcholine (RHC-MPC) hydrogel, (3) arginine-glycine-aspartic acid (RGD) modified silk, and (4) poly-D-lysine (PDL) coated silk, and four electrospun scaffolds: (1) collagen, (2) poly(acrylic acid) (PAA), (3) poly(caprolactone) (PCL), and (4) poly(vinyl alcohol) (PVA). Coverslips and polyethylene terephthalate (PET) were used for comparison. Human conjunctival explants were cultured on scaffolds for 9 to 15 days. Cell viability, outgrowth area, and the percentage of cells expressing markers for stratified squamous epithelial cells (cytokeratin 4) and goblet cells (cytokeratin 7) were determined. RESULTS. Most of cells grown on all scaffolds were viable except for PCL in which only 3.6 +/- 2.2% of the cells were viable. No cells attached to PVA scaffold. The outgrowth was greatest on PDL-silk and PET. Outgrowth was smallest on PCL. All cells were CK7-positive on RHCMPC while 84.7 +/- 6.9% of cells expressed CK7 on PDL-silk. For PCL, 87.10 +/- 3.17% of cells were CK7-positive compared to PET where 67.10 +/- 12.08% of cells were CK7-positive cells. CONCLUSIONS. Biopolymer substrates in the form of hydrogels and silk films provided for better adherence, proliferation, and differentiation than the electrospun scaffolds and could be used for conjunctival goblet cell expansion for eventual transplantation once undifferentiated and stratified squamous cells are included. Useful polymer scaffold design characteristics have emerged from this study.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- polymeric scaffolds; goblet cells; cell growth
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- ref (ämneskategori)
- art (ämneskategori)
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He, Min
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Storr-Paulsen, T ...
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Wang, Annie L.
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Ghezzi, Chiara E ...
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Wang, Siran
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Fullana, Matthew
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Karamichos, Dimi ...
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Utheim, Tor P.
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Islam, Rakibul
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Griffith, May
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Islam, Mohammad ...
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Hodges, Robin R.
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Wnek, Gary E.
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Kaplan, David L.
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Dartt, Darlene A ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Neurovetenskaper
- Artiklar i publikationen
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Investigative Op ...
- Av lärosätet
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Linköpings universitet
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Karolinska Institutet