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Galacturonate Metab...
Galacturonate Metabolism in Anaerobic Chemostat Enrichment Cultures : Combined Fermentation and Acetogenesis by the Dominant sp nov "Candidatus Galacturonibacter soehngenii"
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- Valk, Laura C. (författare)
- Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.
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- Frank, Jeroen (författare)
- Radboud Univ Nijmegen, Soehngen Inst Anaerob Microbiol, Nijmegen, Netherlands.
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- de la Torre-Cortes, Pilar (författare)
- Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.
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- van 't Hof, Max (författare)
- Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.
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- van Maris, Antonius J. A., Professor, 1976- (författare)
- KTH,Industriell bioteknologi,Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.
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- Pronk, Jack T. (författare)
- Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.
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- van Loosdrecht, Mark C. M. (författare)
- Delft Univ Technol, Dept Biotechnol, Delft, Netherlands.
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Delft Univ Technol, Dept Biotechnol, Delft, Netherlands Radboud Univ Nijmegen, Soehngen Inst Anaerob Microbiol, Nijmegen, Netherlands. (creator_code:org_t)
- American Society for Microbiology, 2018
- 2018
- Engelska.
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Ingår i: Applied and Environmental Microbiology. - : American Society for Microbiology. - 0099-2240 .- 1098-5336. ; 84:18
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Agricultural residues such as sugar beet pulp and citrus peel are rich in pectin, which contains galacturonic acid as a main monomer. Pectin-rich residues are underexploited as feedstocks for production of bulk chemicals or biofuels. The anaerobic, fermentative conversion of D-galacturonate in anaerobic chemostat enrichment cultures provides valuable information toward valorization of these pectin-rich feedstocks. Replicate anaerobic chemostat enrichments, with D-galacturonate as the sole limiting carbon source and inoculum from cow rumen content and rotting orange peels, yielded stable microbial communities, which were dominated by a novel Lachnospiraceae species, for which the name "Candidatus Galacturonibacter soehngenii" was proposed. Acetate was the dominant catabolic product, with formate and H-2 as coproducts. The observed molar ratio of acetate and the combined amounts of H-2 and formate deviated significantly from 1, which suggested that some of the hydrogen and CO2 formed during D-galacturonate fermentation was converted into acetate via the Wood-Ljungdahl acetogenesis pathway. Indeed, metagenomic analysis of the enrichment cultures indicated that the genome of "Candidatus G. soehngenii" encoded enzymes of the adapted Entner-Doudoroff pathway for D-galacturonate metabolism as well as enzymes of the Wood-Ljungdahl pathway. The simultaneous operation of these pathways may provide a selective advantage under D-galacturonate-limited conditions by enabling a higher specific ATP production rate and lower residual D-galacturonate concentration than would be possible with a strictly fermentative metabolism of this carbon and energy source. IMPORTANCE This study on D-galacturonate metabolism by open, mixed-culture enrichments under anaerobic, D-galacturonate-limited chemostat conditions shows a stable and efficient fermentation of D-galacturonate into acetate as the dominant organic fermentation product. This fermentation stoichiometry and population analyses provide a valuable baseline for interpretation of the conversion of pectin-rich agricultural feedstocks by mixed microbial cultures. Moreover, the results of this study provide a reference for studies on the microbial metabolism of D-galacturonate under different cultivation regimes.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- acetogenesis
- enrichment culture
- fermentation
- galacturonate
- metagenomics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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