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Cytokine profiles f...
Cytokine profiles for human V gamma 9+ T cells stimulated by Plasmodium falciparum.
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Goodier, M R (författare)
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Lundqvist, C (författare)
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- Hammarström, Marie-Louise (författare)
- Umeå universitet,Immunologi/immunkemi
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Troye-Blomberg, M (författare)
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Langhorne, J (författare)
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(creator_code:org_t)
- 1995
- 1995
- Engelska.
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Ingår i: Parasite immunology (Print). - 0141-9838 .- 1365-3024. ; 17:8, s. 413-23
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- V gamma 9+ T cells from malaria non-exposed donors make proliferative responses to Plasmodium falciparum on in vitro stimulation. V gamma 9+ cells are strongly activated by components of the schizont stage of the parasite and by antigens released into the culture upon schizogony, while CD4+V gamma 9- cells are stimulated by the earlier stages of the parasite. Using reverse transcriptase-polymerase chain reaction (RT-PCR) we determined mRNA expression for 14 cytokines in highly purified V gamma 9+ cells enriched by positive selection after in vitro stimulation with P. falciparum schizont antigens. Interferon-gamma (IFN-gamma) and Tumor Necrosis Factor-alpha (TNF-alpha) were detected in all samples tested. The majority of samples also expressed TNF-beta, transforming growth factor-beta (TGF-beta) and Interleukin-8 (IL-8). Only occasional samples expressed IL-2, IL-5 and IL-10. Using the ELISPOT assay we found that a large fraction of the reactive V gamma 9+ cells produced IFN-gamma and that gamma delta T cells are the major producers of IFN-gamma in cultures stimulated with schizont antigens. The majority of V gamma 9+ cells in these cultures also express the membrane-bound form of TNF-alpha. Expression of these cytokines speaks for a cytolytic and/or inflammatory role of gamma delta cells in the response to malaria in non-exposed individuals.
Ämnesord
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
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