WFRF:(Enerbäck Charlotta 1965 )
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Expression patterns of S100A7 (psoriasin) and S100A9 (calgranulin-B) in keratinocyte differentiation
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- Martinsson, Hanna (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Yhr, Maria, 1968 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Enerbäck, Charlotta, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för dermatologi och venereologi,Institute of Selected Clinical Sciences, Department of Dermatology and Venereology
- (creator_code:org_t)
- 2005
- 2005
- Engelska.
- Ingår i: Exp Dermatol. ; 14:3, s. 161-8
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- S100 proteins are involved in many biological processes. S100A7 and S100A9 have been shown to be markedly upregulated both in ductal carcinoma in situ of the breast and in psoriasis. We have examined the relationship between keratinocyte differentiation and the expression of the two proteins. Using Western blot analysis and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), both S100A7 and S100A9 were shown to be induced in normal primary keratinocytes (HEKn), when differentiation was promoted by high extracellular calcium, loss of contact with extracellular matrix and confluent conditions, as previously reported for S100A7 in mammary epithelial cells. Differentiation was confirmed by using RT-PCR for the differentiation marker keratin-1. Using immunohistochemistry with monoclonal antibodies, we compared the expression of the two proteins in a spectrum of conditions of dysregulated keratinocyte differentiation. We found a strikingly similar distribution of the proteins. Their expression correlated with the degree of keratinocyte differentiation. They were both absent in undifferentiated basalioma and strongly expressed in carcinoma in situ, as well as in keratoacanthoma and differentiated squamous cell carcinoma. In normal epithelium, they were expressed in the superficial, differentiated region of the epithelium rather than in the basal region. These findings support the hypothesis that these two S100 proteins are involved in keratinocyte differentiation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
Nyckelord
- Blotting
- Western
- Calcium-Binding Proteins/*metabolism
- Calgranulin B/*metabolism
- Carcinoma in Situ/metabolism/pathology
- Carcinoma
- Basal Cell/metabolism/pathology
- Carcinoma
- Squamous Cell/metabolism/pathology
- Cell Differentiation/physiology
- Cell Line
- Computer Systems
- Humans
- Immunohistochemistry
- Keratinocytes/*cytology/metabolism/*pathology
- Keratoacanthoma/metabolism/pathology
- Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Skin Neoplasms/metabolism/pathology
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