Leptin stimulates the activity of the system A amino acid transporter in human placental villous fragments.

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Fältnamn	Indikatorer	Metadata
000		04034naa a2200565 4500
001		oai:gup.ub.gu.se/52855
003		SwePub
008		240528s2003 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://gup.ub.gu.se/publication/528552 URI
040		a (SwePub)gu
041		a eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Jansson, Nina,d 1976u Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi,Institute of Physiology and Pharmacology4 aut0 (Swepub:gu)xjanni
245	1 0	a Leptin stimulates the activity of the system A amino acid transporter in human placental villous fragments.
264	1	c 2003
520		a The activity and expression of placental nutrient transporters are primary determinants for the supply of nutrients to the fetus, and these nutrients in turn regulate fetal growth. We developed an experimental system to assess amino acid uptake in single primary villous fragments to study hormonal regulation of the amino acid transporter system A in term human placenta. Validation of the method, using electron microscopy and studies of hormone production, indicated that fragments maintained ultrastructural and functional integrity for at least 3 h. The activity of system A was measured as the Na(+)-dependent uptake of methylaminoisobutyric acid (MeAIB), and the effect of 1 h incubation in various hormones was investigated. Uptake of MeAIB into villous fragments in the presence of Na(+) was linear up to at least 30 min. Insulin (300 ng/ml, n = 14) increased system A activity by 56% (P < 0.05). This effect was also present at insulin concentrations in the physiological range (+47% at 0.6 ng/ml, n = 10, P < 0.05). Leptin (500 ng/ml, n = 14) increased Na(+)-dependent MeAIB uptake by 37% (P < 0.05). System A activity increased in a concentration-dependent fashion in response to leptin (n = 10). However, neither epidermal GF (600 ng/ml), cortisol (340 ng/ml), nor GH (500 ng/ml) altered system A activity significantly (n = 14). We conclude that primary single isolated villous fragments can be used in studies of hormonal regulation of nutrient uptake into the syncytiotrophoblast. These data suggest that leptin regulates system A, a key amino acid transporter.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Fysiologi0 (SwePub)301062 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Physiology0 (SwePub)301062 hsv//eng
653		a Amino Acid Transport System A
653		a drug effects
653		a Amino Acids
653		a metabolism
653		a Female
653		a Humans
653		a L-Lactate Dehydrogenase
653		a secretion
653		a Leptin
653		a pharmacology
653		a Microscopy
653		a Electron
653		a Microvilli
653		a metabolism
653		a Placenta
653		a metabolism
653		a ultrastructure
653		a Pregnancy
653		a Sodium
653		a metabolism
653		a beta-Alanine
653		a analogs & derivatives
653		a metabolism
700	1	a Greenwood, S L4 aut
700	1	a Johansson, Bengt R,d 1947u Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology4 aut0 (Swepub:gu)xjbenp
700	1	a Powell, Theresa L,d 1959u Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi,Institute of Physiology and Pharmacology4 aut0 (Swepub:gu)xpowth
700	1	a Jansson, Thomas,d 1955u Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi,Institute of Physiology and Pharmacology4 aut0 (Swepub:gu)xjanst
710	2	a Göteborgs universitetb Institutionen för fysiologi och farmakologi4 org
773	0	t The Journal of clinical endocrinology and metabolismg 88:3, s. 1205-11q 88:3<1205-11x 0021-972X
856	4 8	u https://gup.ub.gu.se/publication/52855

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