The rat antigen-presenting lectin-like receptor complex influences innate immunity and development of infectious diseases.

• Genetic variation in the antigen-presenting lectin-like receptor gene complex (APLEC) associates with autoimmunity and arthritis in rats and humans. We hypothesized that the encoded C-type lectin-like receptors might influence innate immunity and responses to infectious agents. To test this hypothesis, we compared in vivo and in vitro phenotypes in DA rats and APLEC-congenic rats. Survival rates following infection with Staphylococcus aureus and Herpes simplex virus differed significantly between the two strains. Likewise, differential delayed type hypersensitivity (DTH), an immunological reaction involving T lymphocytes and macrophages, was observed in response to provocation with the chemical oxazolone. Unstimulated bone marrow-derived macrophages from the two strains appeared to already have polarized activation states with different mRNA levels of CD163 and Dectin-1 receptors. Following stimulation with a panel of microbial agents, differences in induced mRNA and protein levels were shown for interleukin (IL)-6 and IL-10 following stimulation with lipopolysaccharide, mannan and beta-glucan. Expression levels of APLEC gene mRNAs also differed, and both strains had a notably dichotomous expression of the genes, with general downregulation of all four Dcir genes and upregulation of Mincle and Mcl. We suggest that human APLEC genes may similarly regulate infectious diseases, DTH and general macrophage activation status.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Keyword

Adjuvants

Immunologic

pharmacology

Animals

Arthritis

Infectious

genetics

immunology

microbiology

mortality

Cells

Cultured

Communicable Diseases

genetics

immunology

Cytokines

biosynthesis

genetics

immunology

Encephalitis

genetics

immunology

virology

Herpesvirus 1

Human

immunology

Hypersensitivity

Delayed

genetics

immunology

Immunity

Innate

Lectins

C-Type

genetics

immunology

Lipopolysaccharides

pharmacology

Macrophage Activation

drug effects

genetics

immunology

Macrophages

immunology

metabolism

microbiology

virology

Mannans

pharmacology

Oxazolone

pharmacology

Rats

Staphylococcal Infections

genetics

immunology

Staphylococcus aureus

immunology

Zymosan

pharmacology

beta-Glucans

pharmacology

Publication and Content Type

ref (subject category)

art (subject category)