Application of Artificial Gel Antibodies for the Detection and Quantification of Proteins in Biological Fluids

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Fältnamn	Indikatorer	Metadata
000		04132nam a2200409 4500
001		oai:DiVA.org:uu-122457
003		SwePub
008		100413s2010 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
020		a 9789155478025q print
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1224572 URI
040		a (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a vet2 swepub-contenttype
072	7	a dok2 swepub-publicationtype
100	1	a Ghasemzadeh, Nasimu Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Fred Nyberg4 aut0 (Swepub:uu)nagha254
245	1 0	a Application of Artificial Gel Antibodies for the Detection and Quantification of Proteins in Biological Fluids
264	1	a Uppsala :b Acta Universitatis Upsaliensis,c 2010
300		a 64 s.
338		a electronic2 rdacarrier
490	0	a Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology,x 1651-6214 ;v 741
520		a The molecular-imprinting method has previously been used for the synthesis of artificial gel antibodies, highly selective for various proteins. In present study, we have synthesized artificial gel antibodies against haemoglobin, albumin and different forms of growth hormone with the aim to develop a simple and rapid procedure to measure the concentration of these protein biomarkers in samples of clinical interest. A spectrophotometric method was developed to design a standard curve in the form of a straight line, whereby the true absorption (not the recorded “apparent” absorption) was plotted against a known protein concentration. The procedure, applied to quantitative analysis of albumin in human plasma and cerebrospinal fluid (CSF) from patients with ALS, indicated that the concentration of this protein was significantly enhanced in CSF from patients with amyotrophic lateral sclerosis (ALS), compared to control samples. A low level of albumin was observed in plasma from ALS patients compared to controls. Additionally, free zone electrophoresis was employed to detect human growth hormone (GH) activity in hormone preparations purified from human pituitaries. We have successfully synthesized antibodies capable of discriminating between dimeric and monomeric GH in samples of clinical origin. To quantify these proteins a calibration curve has been designed, i.e. a plot of the electrophoretic mobility of the complex GH/gel antibody against the protein concentration in the sample, for instance serum or CSF. This method was also employed for qualitative and quantitative determinations of Somatropin, a non-glycosylated GH and glycosylated-GH in a body liquid. Our results indicate that by this technique one can “fish out” with high accuracy various proteins from both body fluids containing a great number of other proteins. It might well apply also to biomarker proteins for other diseases.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Läkemedelskemi0 (SwePub)301032 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Medicinal Chemistry0 (SwePub)301032 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Farmaceutiska vetenskaper0 (SwePub)301012 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Pharmaceutical Sciences0 (SwePub)301012 hsv//eng
653		a Pharmaceutical chemistry
653		a Farmaceutisk kemi
653		a Pharmaceutical biochemistry
653		a Farmaceutisk biokemi
653		a Farmaceutisk vetenskap
653		a Pharmaceutical Science
700	1	a Nyberg, Fred,c Prof.u Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Avdelningen för biologisk beroendeforskning4 ths
700	1	a Desiderio, Dom M.,c Prof.u Charles B. Stout Neuroscience Mass Spectrometry Laboratory4 opn
710	2	a Uppsala universitetb Institutionen för farmaceutisk biovetenskap4 org
856	4	u https://uu.diva-portal.org/smash/get/diva2:310187/FULLTEXT01.pdfx primaryx Raw objecty fulltext
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-122457

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