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The importance of t...
The importance of tryptic-like activity in purified enzyme blends for efficient islet isolation
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- Brandhorst, Heide, 1962- (author)
- Uppsala universitet,Enheten för klinisk immunologi,Olle Korsgren
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- Friberg, Andrew (author)
- Uppsala universitet,Enheten för klinisk immunologi,Olle Korsgren
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Andersson, Helena H. (author)
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Felldin, Maria (author)
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Foss, Aksel (author)
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Salmela, Kaija (author)
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- Lundgren, Torbjörn (author)
- Karolinska Institutet
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- Tibell, Annika (author)
- Karolinska Institutet
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- Tufveson, Gunnar (author)
- Uppsala universitet,Transplantationskirurgi
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- Korsgren, Olle (author)
- Uppsala universitet,Enheten för klinisk immunologi,Olle Korsgren
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- Brandhorst, Daniel, 1961- (author)
- Uppsala universitet,Enheten för klinisk immunologi,Olle Korsgren
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(creator_code:org_t)
- 2009
- 2009
- English.
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In: Transplantation. - 0041-1337 .- 1534-6080. ; 87:3, s. 370-5
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- BACKGROUND: The isolation of islets from the human pancreas critically depends on an efficient enzyme blend. Previous studies have solely focused on the presence of collagenase and neutral protease/thermolysin. Despite improved characterization of these components, the lot-related variability in efficacy still persists suggesting that additional so far disregarded enzymes are required for efficient islet cleavage. METHODS: Varying activities of a tryptic-like enzyme were identified within collagenase NB1 lots, which were selected according to a matched ratio between tryptic-like and collagenase activity (TLA-ratio). Rat and human pancreata were processed with current standard procedures. RESULTS: Increasing the TLA-ratio from 1.3% to 10% reduced pancreas dissociation time in rats by 50% without affecting islet yield, viability, or posttransplant function in diabetic nude mice. Enhancing the TLA-ratio from 1.3% to 12.6% for human pancreas processing resulted in a significant reduction of recirculation time and increased incrementally human islet yield without affecting purity, in vitro function or recovery after culture. Optimized pancreas digestion correlated with a higher percentage of islet preparations fulfilling quality criteria for clinical transplantation. CONCLUSIONS: We conclude that TLA is an effective component that should be included in moderate amounts in enzyme blends for human islet isolation to optimize the efficiency and minimize the lot-related variability.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Keyword
- human islet transplantation
- human islet isolation
- collagenase
- enzymes
- Transplantation surgery
- Transplantationskirurgi
- Medical Science
- Medicinsk vetenskap
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Brandhorst, Heid ...
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Friberg, Andrew
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Andersson, Helen ...
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Felldin, Maria
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Foss, Aksel
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Salmela, Kaija
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show more...
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Lundgren, Torbjö ...
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Tibell, Annika
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Tufveson, Gunnar
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Korsgren, Olle
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Brandhorst, Dani ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Surgery
- Articles in the publication
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Transplantation
- By the university
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Uppsala University
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Karolinska Institutet