Data-driven microscopy allows for automated context-specific acquisition of high-fidelity image data

André, Oscar (author): Lund University,Lunds universitet,Kvantitativ immunbiologi,Forskargrupper vid Lunds universitet,Quantitative immunobiology,Lund University Research Groups

Kumra Ahnlide, Johannes (author): Lund University,Lunds universitet,Kvantitativ immunbiologi,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Quantitative immunobiology,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments

Norlin, Nils (author): Lund University,Lunds universitet,Molekylär neuromodulering,Forskargrupper vid Lunds universitet,Lund University Bioimaging Center,Medicinska fakulteten,LTH profilområde: Teknik för hälsa,LTH profilområden,Lunds Tekniska Högskola,Molecular Neuromodulation,Lund University Research Groups,Faculty of Medicine,LTH Profile Area: Engineering Health,LTH Profile areas,Faculty of Engineering, LTH

Swaminathan, Vinay (author): Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,WCMM- Wallenberg center för molekylär medicinsk forskning,Medicinska fakulteten,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Cellmekanobiologi,Sektion I,Institutionen för kliniska vetenskaper, Lund,LTH profilområde: Nanovetenskap och halvledarteknologi,LTH profilområden,LTH profilområde: Avancerade ljuskällor,LU profilområde: Ljus och material,Lunds universitets profilområden,Other operations, LTH,Faculty of Engineering, LTH,WCMM-Wallenberg Centre for Molecular Medicine,Faculty of Medicine,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Cell mechanobiology,Section I,Department of Clinical Sciences, Lund,LTH Profile Area: Nanoscience and Semiconductor Technology,LTH Profile areas,Faculty of Engineering, LTH,LTH Profile Area: Photon Science and Technology,Faculty of Engineering, LTH,LU Profile Area: Light and Materials,Lund University Profile areas

Nordenfelt, Pontus (author): Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Kvantitativ immunbiologi,Forskargrupper vid Lunds universitet,SEBRA Sepsis and Bacterial Resistance Alliance,epIgG,LTH profilområde: Nanovetenskap och halvledarteknologi,LTH profilområden,LTH profilområde: Avancerade ljuskällor,LU profilområde: Ljus och material,Lunds universitets profilområden,Other operations, LTH,Faculty of Engineering, LTH,Quantitative immunobiology,Lund University Research Groups,LTH Profile Area: Nanoscience and Semiconductor Technology,LTH Profile areas,Faculty of Engineering, LTH,LTH Profile Area: Photon Science and Technology,Faculty of Engineering, LTH,LU Profile Area: Light and Materials,Lund University Profile areas

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Abstract Subject headings

Light microscopy is a powerful single-cell technique that allows for quantitative spatial information at subcellular resolution. However, unlike flow cytometry and single-cell sequencing techniques, microscopy has issues achieving high-quality population-wide sample characterization while maintaining high resolution. Here, we present a general framework, data-driven microscopy (DDM) that uses real-time population-wide object characterization to enable data-driven high-fidelity imaging of relevant phenotypes based on the population context. DDM combines data-independent and data-dependent steps to synergistically enhance data acquired using different imaging modalities. As a proof of concept, we develop and apply DDM with plugins for improved high-content screening and live adaptive microscopy for cell migration and infection studies that capture events of interest, rare or common, with high precision and resolution. We propose that DDM can reduce human bias, increase reproducibility, and place single-cell characteristics in the context of the sample population when interpreting microscopy data, leading to an increase in overall data fidelity.

By the author/editor: André, Oscar; Kumra Ahnlide, J ...; Norlin, Nils; Swaminathan, Vin ...; Nordenfelt, Pont ...

About the subject

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Medical Engineer ...; and Medical Image Pr ...

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