Sökning: WFRF:(Antonsson K) > Clonal culturing of...
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000 | 03120naa a2200517 4500 | |
001 | oai:prod.swepub.kib.ki.se:128264658 | |
003 | SwePub | |
008 | 240701s2014 | |||||||||||000 ||eng| | |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1282646582 URI |
024 | 7 | a https://doi.org/10.1038/ncomms41952 DOI |
040 | a (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Rodin, Su Karolinska Institutet4 aut |
245 | 1 0 | a Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment |
264 | c 2014-01-27 | |
264 | 1 | b Springer Science and Business Media LLC,c 2014 |
520 | a Lack of robust methods for establishment and expansion of pluripotent human embryonic stem (hES) cells still hampers development of cell therapy. Laminins (LN) are a family of highly cell-type specific basement membrane proteins important for cell adhesion, differentiation, migration and phenotype stability. Here we produce and isolate a human recombinant LN-521 isoform and develop a cell culture matrix containing LN-521 and E-cadherin, which both localize to stem cell niches in vivo. This matrix allows clonal derivation, clonal survival and long-term self-renewal of hES cells under completely chemically defined and xeno-free conditions without ROCK inhibitors. Neither LN-521 nor E-cadherin alone enable clonal survival of hES cells. The LN-521/E-cadherin matrix allows hES cell line derivation from blastocyst inner cell mass and single blastomere cells without a need to destroy the embryo. This method can facilitate the generation of hES cell lines for development of different cell types for regenerative medicine purposes. | |
700 | 1 | a Antonsson, Lu Karolinska Institutet4 aut |
700 | 1 | a Niaudet, C4 aut |
700 | 1 | a Simonson, OE4 aut |
700 | 1 | a Salmela, E4 aut |
700 | 1 | a Hansson, EM4 aut |
700 | 1 | a Domogatskaya, Au Karolinska Institutet4 aut |
700 | 1 | a Xiao, ZJ4 aut |
700 | 1 | a Damdimopoulou, Pu Karolinska Institutet4 aut |
700 | 1 | a Sheikhi, M4 aut |
700 | 1 | a Inzunza, Ju Karolinska Institutet4 aut |
700 | 1 | a Nilsson, ASu Karolinska Institutet4 aut |
700 | 1 | a Baker, D4 aut |
700 | 1 | a Kuiper, Ru Karolinska Institutet4 aut |
700 | 1 | a Sun, Y4 aut |
700 | 1 | a Blennow, Eu Karolinska Institutet4 aut |
700 | 1 | a Nordenskjold, Mu Karolinska Institutet4 aut |
700 | 1 | a Grinnemo, KHu Karolinska Institutet4 aut |
700 | 1 | a Kere, Ju Karolinska Institutet4 aut |
700 | 1 | a Betsholtz, Cu Karolinska Institutet4 aut |
700 | 1 | a Hovatta, Ou Karolinska Institutet4 aut |
700 | 1 | a Tryggvason, Ku Karolinska Institutet4 aut |
710 | 2 | a Karolinska Institutet4 org |
773 | 0 | t Nature communicationsd : Springer Science and Business Media LLCg 5, s. 3195-q 5<3195-x 2041-1723 |
856 | 4 | u https://www.nature.com/articles/ncomms4195.pdf |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:128264658 |
856 | 4 8 | u https://doi.org/10.1038/ncomms4195 |
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