Major signal suppression from metal ion clusters in SFC/ESI-MS: Cause and Effects

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Fältnamn	Indikatorer	Metadata
000		03902naa a2200409 4500
001		oai:DiVA.org:uu-345978
003		SwePub
008		180313s2018 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3459782 URI
024	7	a https://doi.org/10.1016/j.jchromb.2018.03.0242 DOI
040		a (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Haglind, Alfred,d 1986-u Uppsala universitet,Institutionen för läkemedelskemi4 aut0 (Swepub:uu)alfsv446
245	1 0	a Major signal suppression from metal ion clusters in SFC/ESI-MS :b Cause and Effects
264	1	b Elsevier BV,c 2018
338		a print2 rdacarrier
520		a The widening application area of SFC-MS with polar analytes and water-containing samples facilitates the use of quick and simple sample preparation techniques such as “dilute and shoot” and protein precipitation. This has also introduced new polar interfering components such as alkali metal ions naturally abundant in e.g. blood plasma and urine, which have shown to be retained using screening conditions in SFC/ESI-TOF-MS and causing areas of major ion suppression. Analytes co-eluting with these clusters will have a decreased signal intensity, which might have a major effect on both quantification and identification. When investigating the composition of the alkali metal clusters using accurate mass and isotopic pattern, it could be concluded that they were previously not described in the literature. Using NaCl and KCl standards and different chromatographic conditions, varying e.g. column and modifier, the clusters proved to be formed from the alkali metal ions in combination with the alcohol modifier and make-up solvent. Their compositions were [(XOCH3)n+X]+, [(XOH)n+X]+, [(X2CO3)n+X]+ and [(XOOCOCH3)n+X]+ for X= Na+ or K+ in ESI+. In ESI-, the clusters depended more on modifier, with [(XCl)n+Cl]- and [(XOCH3)n+OCH3]- mainly formed in pure methanol and [(XOOCH)n+OOCH]- when 20 mM NH4Fa was added.To prevent the formation of the clusters by avoiding methanol as modifier might be difficult, as this is a widely used modifier providing good solubility when analyzing polar compounds in SFC. A sample preparation with e.g. LLE would remove the alkali ions, however also introducing a time consuming and discriminating step into the method. Since the alkali metal ions were retained and affected by chromatographic adjustments as e.g. mobile phase modifications, a way to avoid them could therefore be chromatographic tuning, when analyzing samples containing them.
650	7	a NATURVETENSKAPx Kemix Analytisk kemi0 (SwePub)104012 hsv//swe
650	7	a NATURAL SCIENCESx Chemical Sciencesx Analytical Chemistry0 (SwePub)104012 hsv//eng
653		a SFC-MS
653		a matrix effect
653		a alkali metal
653		a ion cluster
653		a Supercritical fluid chromatography
653		a ESI
653		a Analytical Pharmaceutical Chemistry
653		a Analytisk farmaceutisk kemi
700	1	a Hedeland, Mikaelu Uppsala universitet,Institutionen för läkemedelskemi,National Veterinary Institute (SVA), Dept. of Chemistry, Environment and Feed Hygiene, Uppsala, Sweden4 aut0 (Swepub:uu)mikahede
700	1	a Arvidsson, Torbjörnu Uppsala universitet,Institutionen för läkemedelskemi,Medical Products Agency, Uppsala, Sweden4 aut0 (Swepub:uu)toarv517
700	1	a Pettersson, Curt Eu Uppsala universitet,Institutionen för läkemedelskemi4 aut0 (Swepub:uu)curtps
710	2	a Uppsala universitetb Institutionen för läkemedelskemi4 org
773	0	t Journal of chromatography. Bd : Elsevier BVg 1084, s. 96-105q 1084<96-105x 1570-0232x 1873-376X
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-345978
856	4 8	u https://doi.org/10.1016/j.jchromb.2018.03.024

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