WFRF:(Hemmer P. H. J.)
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 06465naa a2201321 4500 | |
| 001 | oai:DiVA.org:uu-122997 | |
| 003 | SwePub | |
| 008 | 100422s2010 | |||||||||||000 ||eng| | |
| 009 | oai:DiVA.org:umu-41961 | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1229972 URI |
| 024 | 7 | a https://doi.org/10.1016/j.jim.2009.09.0142 DOI |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-419612 URI |
| 040 | a (SwePub)uud (SwePub)umu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Petzold, Axel4 aut |
| 245 | 1 0 | a Neurofilament ELISA validation |
| 264 | 1 | b Elsevier BV,c 2010 |
| 338 | a print2 rdacarrier | |
| 520 | a BACKGROUND: Neurofilament proteins (Nf) are highly specific biomarkers for neuronal death and axonal degeneration. As these markers become more widely used, an inter-laboratory validation study is required to identify assay criteria for high quality performance. METHODS: The UmanDiagnostics NF-light (R)enzyme-linked immunoabsorbent assays (ELISA) for the neurofilament light chain (NfL, 68kDa) was used to test the intra-assay and inter-laboratory coefficient of variation (CV) between 35 laboratories worldwide on 15 cerebrospinal fluid (CSF) samples. Critical factors, such as sample transport and storage, analytical delays, reaction temperature and time, the laboratories' accuracy and preparation of standards were documented and used for the statistical analyses. RESULTS: The intra-laboratory CV averaged 3.3% and the inter-laboratory CV 59%. The results from the test laboratories correlated with those from the reference laboratory (R=0.60, p<0.0001). Correcting for critical factors improved the strength of the correlation. Differences in the accuracy of standard preparation were identified as the most critical factor. Correcting for the error introduced by variation in the protein standards improved the correlation to R=0.98, p<0.0001 with an averaged inter-laboratory CV of 14%. The corrected overall inter-rater agreement was subtantial (0.6) according to Fleiss' multi-rater kappa and Gwet's AC1 statistics. CONCLUSION: This multi-center validation study identified the lack of preparation of accurate and consistent protein standards as the main reason for a poor inter-laboratory CV. This issue is also relevant to other protein biomarkers based on this type of assay and will need to be solved in order to achieve an acceptable level of analytical accuracy. The raw data of this study is available online. | |
| 653 | a Neurofilament | |
| 653 | a NfL | |
| 653 | a NF-L | |
| 653 | a NEFL | |
| 653 | a Biomarker | |
| 653 | a Validation | |
| 653 | a MEDICINE | |
| 653 | a MEDICIN | |
| 700 | 1 | a Altintas, Ayse4 aut |
| 700 | 1 | a Andreoni, Laura4 aut |
| 700 | 1 | a Bartos, Ales4 aut |
| 700 | 1 | a Berthele, Achim4 aut |
| 700 | 1 | a Blankenstein, Marinus A4 aut |
| 700 | 1 | a Buee, Luc4 aut |
| 700 | 1 | a Castellazzi, Massimiliano4 aut |
| 700 | 1 | a Cepok, Sabine4 aut |
| 700 | 1 | a Comabella, Manuel4 aut |
| 700 | 1 | a Constantinescu, Cris S4 aut |
| 700 | 1 | a Deisenhammer, Florian4 aut |
| 700 | 1 | a Deniz, Gunnur4 aut |
| 700 | 1 | a Erten, Gaye4 aut |
| 700 | 1 | a Espiño, Mercedes4 aut |
| 700 | 1 | a Fainardi, Enrico4 aut |
| 700 | 1 | a Franciotta, Diego4 aut |
| 700 | 1 | a Freedman, Mark S4 aut |
| 700 | 1 | a Giedraitis, Vilmantasu Uppsala universitet,Geriatrik4 aut0 (Swepub:uu)vigie517 |
| 700 | 1 | a Gilhus, Nils Erik4 aut |
| 700 | 1 | a Giovannoni, Gavin4 aut |
| 700 | 1 | a Glabinski, Andrzej4 aut |
| 700 | 1 | a Grieb, Pawel4 aut |
| 700 | 1 | a Hartung, Hans-Peter4 aut |
| 700 | 1 | a Hemmer, Bernhard4 aut |
| 700 | 1 | a Herukka, Sanna-Kaisa4 aut |
| 700 | 1 | a Hintzen, Rogier4 aut |
| 700 | 1 | a Ingelsson, Martinu Uppsala universitet,Geriatrik4 aut0 (Swepub:uu)maing121 |
| 700 | 1 | a Jackson, Samuel4 aut |
| 700 | 1 | a Jacobsen, Steve4 aut |
| 700 | 1 | a Jafari, Naghmeh4 aut |
| 700 | 1 | a Jalosinski, Marcin4 aut |
| 700 | 1 | a Jarius, Sven4 aut |
| 700 | 1 | a Kapaki, Elisabeth4 aut |
| 700 | 1 | a Kieseier, Bernd C4 aut |
| 700 | 1 | a Koel-Simmelink, Marleen J A4 aut |
| 700 | 1 | a Kornhuber, Johannes4 aut |
| 700 | 1 | a Kuhle, Jens4 aut |
| 700 | 1 | a Kurzepa, Jacek4 aut |
| 700 | 1 | a Lalive, Patrice H4 aut |
| 700 | 1 | a Lannfelt, Larsu Uppsala universitet,Geriatrik4 aut0 (Swepub:uu)lalan021 |
| 700 | 1 | a Lehmensiek, Vera4 aut |
| 700 | 1 | a Lewczuk, Piotr4 aut |
| 700 | 1 | a Livrea, Paolo4 aut |
| 700 | 1 | a Marnetto, Fabiana4 aut |
| 700 | 1 | a Martino, Davide4 aut |
| 700 | 1 | a Menge, Til4 aut |
| 700 | 1 | a Norgren, Niklasu UmanDiagnostics, Umea Biotech Incubator, Tvistevägen 48, SE-90736 Umea, Sweden4 aut0 (Swepub:umu)nisnon97 |
| 700 | 1 | a Papuć, Eva4 aut |
| 700 | 1 | a Paraskevas, George P4 aut |
| 700 | 1 | a Pirttilä, Tuula4 aut |
| 700 | 1 | a Rajda, Cecília4 aut |
| 700 | 1 | a Rejdak, Konrad4 aut |
| 700 | 1 | a Ricny, Jan4 aut |
| 700 | 1 | a Ripova, Daniela4 aut |
| 700 | 1 | a Rosengren, Lars4 aut |
| 700 | 1 | a Ruggieri, Maddalena4 aut |
| 700 | 1 | a Schraen, Susanna4 aut |
| 700 | 1 | a Shaw, Gerry4 aut |
| 700 | 1 | a Sindic, Christian4 aut |
| 700 | 1 | a Siva, Aksel4 aut |
| 700 | 1 | a Stigbrand, Torgnyu Umeå universitet,Immunologi/immunkemi,Stigbrand4 aut0 (Swepub:umu)tost0001 |
| 700 | 1 | a Stonebridge, Iva4 aut |
| 700 | 1 | a Topcular, Baris4 aut |
| 700 | 1 | a Trojano, Maria4 aut |
| 700 | 1 | a Tumani, Hayrettin4 aut |
| 700 | 1 | a Twaalfhoven, Harry A M4 aut |
| 700 | 1 | a Vécsei, László4 aut |
| 700 | 1 | a Van Pesch, Vincent4 aut |
| 700 | 1 | a Vanderstichele, Hugo4 aut |
| 700 | 1 | a Vedeler, Christian4 aut |
| 700 | 1 | a Verbeek, Marcel M4 aut |
| 700 | 1 | a Villar, Luisa Maria4 aut |
| 700 | 1 | a Weissert, Robert4 aut |
| 700 | 1 | a Wildemann, Brigitte4 aut |
| 700 | 1 | a Yang, Cui4 aut |
| 700 | 1 | a Yao, Karen4 aut |
| 700 | 1 | a Teunissen, Charlotte E4 aut |
| 710 | 2 | a Uppsala universitetb Geriatrik4 org |
| 773 | 0 | t JIM - Journal of Immunological Methodsd : Elsevier BVg 352:1-2, s. 23-31q 352:1-2<23-31x 0022-1759x 1872-7905 |
| 856 | 4 | u https://discovery.ucl.ac.uk/95615/1/nf-validation-PR-v6.pdf |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-122997 |
| 856 | 4 8 | u https://doi.org/10.1016/j.jim.2009.09.014 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-41961 |
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