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Single-cell electro...
Single-cell electroporation
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- Wang, M. Y. (författare)
- University of Pittsburgh
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- Orwar, Owe, 1964 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Olofsson, Jessica, 1975 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Weber, S. G. (författare)
- University of Pittsburgh
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(creator_code:org_t)
- 2010-05-23
- 2010
- Engelska.
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Ingår i: Analytical and Bioanalytical Chemistry. - : Springer Science and Business Media LLC. - 1618-2642 .- 1618-2650. ; 397:8, s. 3235-3248
- Relaterad länk:
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http://dx.doi.org/10...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Single-cell electroporation (SCEP) is a relatively new technique that has emerged in the last decade or so for single-cell studies. When a large enough electric field is applied to a single cell, transient nano-pores form in the cell membrane allowing molecules to be transported into and out of the cell. Unlike bulk electroporation, in which a homogenous electric field is applied to a suspension of cells, in SCEP an electric field is created locally near a single cell. Today, single-cell-level studies are at the frontier of biochemical research, and SCEP is a promising tool in such studies. In this review, we discuss pore formation based on theoretical and experimental approaches. Current SCEP techniques using microelectrodes, micropipettes, electrolyte-filled capillaries, and microfabricated devices are all thoroughly discussed for adherent and suspended cells. SCEP has been applied in in-vivo and in-vitro studies for delivery of cell-impermeant molecules such as drugs, DNA, and siRNA, and for morphological observations.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Transfection
- patch-clamp
- filled capillary
- Cell membrane
- Electric field
- modeling electroporation
- electric-field
- Microelectrodes
- genetic manipulation
- mammalian-cells
- Microfluidics
- neurons in-vivo
- membrane electropermeabilization
- ellipsoidal cells
- micro-electroporation
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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