Increased gene expression of novel cytosolic and secretory phospholipase A2 types in human airway epithelial cells induced by tumor necrosis factor-α and IFN-γ

Lindbom, John, 1960- (author): Linköpings universitet,Yrkes- och miljömedicin,Hälsouniversitetet

Ljungman, Anders, 1958- (author): Linköpings universitet,Yrkes- och miljömedicin,Hälsouniversitetet

Lindahl, Mats, 1957- (author): Linköpings universitet,Yrkes- och miljömedicin,Hälsouniversitetet

Tagesson, Christer, 1948- (author): Linköpings universitet,Yrkes- och miljömedicin,Hälsouniversitetet

(creator_code:org_t)

Mary Ann Liebert Inc, 2002
2002
English.
In: Journal of Interferon and Cytokine Research. - : Mary Ann Liebert Inc. - 1079-9907 .- 1557-7465. ; 22:9, s. 947-955

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

Phospholipase A2 (PLA2) is a growing family of enzymes that may play a major role in inflammation. We investigated the effect of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) on the gene expression of 19 different PLA2 types (IB, IIA, IID, IIE, IIF, III, IVA, IVB, IVC, V, VIA, VIB, VIIA, VIIB, VIIIA, VIIIB, X, XII, and XIII) in human bronchoepithelial (BEAS-2B) and nasal epithelial (RPMI 2650) cells. The cells were stimulated with TNF-α or IFN-γ for different lengths of time (1, 4, 18, and 48 h), and the mRNA levels of the different PLA2 types were determined by reverse transcriptase-PCR (RT-PCR) and normalized to those of the housekeeping gene, GAPDH. In both cell lines, TNF-α increased the expression of PLA2 IVA and IVC, and IFN-γ increased the expression of PLA2 IIA and IID. No influence on the gene expression of PLA2-activating protein (PLAP) was noted on cytokine stimulation. These findings indicate that TNF-α and IFN-γ induce gene expression of two novel cytosolic and secretory PLA2 types (IVC and IID, respectively) in human airway epithelial cells. The possibility that these PLA2 types are involved in cytokine-mediated inflammation in the respiratory tract is inferred.

By the author/editor: Lindbom, John, 1 ...; Ljungman, Anders ...; Lindahl, Mats, 1 ...; Tagesson, Christ ...

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