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C57BL/6 mice need M...
C57BL/6 mice need MHC class II Aq to develop collagen-induced arthritis dependent on autoreactive T cells
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- Backlund, J. (författare)
- Karolinska Institutet,Karolinska Institute, Stockholm, Sweden
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- Li, C. (författare)
- Karolinska Institute, Stockholm, Sweden
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- Jansson, E. (författare)
- Karolinska Institute, Stockholm, Sweden
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- Carlsen, S. (författare)
- Karolinska Institute, Stockholm, Sweden
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- Merky, P. (författare)
- Karolinska Institutet,Karolinska Institute, Stockholm, Sweden
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- Nandakumar, Kutty Selva, 1965- (författare)
- Karolinska Institutet,Karolinska Institute, Stockholm, Sweden
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- Haag, S. (författare)
- Karolinska Institutet,Karolinska Institute, Stockholm, Sweden
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- Ytterberg, J. (författare)
- Karolinska Institutet,Karolinska University Hospital, Stockholm, Sweden
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- Zubarev, R. A. (författare)
- Karolinska Institutet,Karolinska Institute, Stockholm, Sweden
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- Holmdahl, R. (författare)
- Karolinska Institutet,Karolinska Institute, Stockholm, Sweden
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(creator_code:org_t)
- 2012-10-05
- 2013
- Engelska.
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Ingår i: Annals of the Rheumatic Diseases. - : BMJ. - 0003-4967 .- 1468-2060. ; 72:7, s. 1225-1232
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- INTRODUCTION: Collagen-induced arthritis (CIA) has traditionally been performed in MHC class II A(q)-expressing mice, whereas most genetically modified mice are on the C57BL/6 background (expressing the b haplotype of the major histocompatibility complex (MHC) class II region). However, C57BL/6 mice develop arthritis after immunisation with chicken-derived collagen type II (CII), but arthritis susceptibility has been variable, and the immune specificity has not been clarified. OBJECTIVE: To establish a CIA model on the C57BL/6 background with a more predictable and defined immune response to CII. RESULTS: Both chicken and rat CII were arthritogenic in C57BL/6 mice provided they were introduced with high doses of Mycobacterium tuberculosis adjuvant. However, contaminating pepsin was strongly immunogenic and was essential for arthritis development. H-2(b)-restricted T cell epitopes on chicken or rat CII could not be identified, but expression of A(q) on the C57BL/6 background induced T cell response to the CII260-270 epitope, and also prolonged the arthritis to be more chronic. CONCLUSIONS: The putative (auto)antigen and its arthritogenic determinants in C57BL/6 mice remains undisclosed, questioning the value of the model for addressing T cell-driven pathological pathways in arthritis. To circumvent this impediment, we recommend MHC class II congenic C57BL/6N.Q mice, expressing A(q), with which T cell determinants have been thoroughly characterised.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
Nyckelord
- Animals
- Arthritis
- Experimental/*genetics/immunology
- Arthritis
- Rheumatoid/*genetics/immunology
- Chickens
- Collagen Type II/*immunology
- *Disease Models
- Animal
- Epitopes
- T-Lymphocyte/immunology
- Genes
- MHC Class II/*genetics
- Haplotypes
- Immunization
- Mice
- Mice
- Congenic
- Mice
- Inbred C57BL
- Mice
- Inbred Strains
- Mycobacterium/immunology
- Rats
- T-Lymphocytes/*immunology
- Autoimmune Diseases
- Inflammation
- T Cells
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Backlund, J.
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Li, C.
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Jansson, E.
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Carlsen, S.
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Merky, P.
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Nandakumar, Kutt ...
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visa fler...
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Haag, S.
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Ytterberg, J.
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Zubarev, R. A.
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Holmdahl, R.
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visa färre...
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Högskolan i Halmstad
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Karolinska Institutet