Development of a Molecular Imprinting-Based Surface Plasmon Resonance Biosensor for Rapid and Sensitive Detection of Staphylococcus aureus Alpha Hemolysin From Human Serum

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Fältnamn	Indikatorer	Metadata
000		04314naa a2200421 4500
001		oai:lup.lub.lu.se:32ae4433-593f-4fce-b497-44204f0fcb25
003		SwePub
008		201215s2020 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://lup.lub.lu.se/record/32ae4433-593f-4fce-b497-44204f0fcb252 URI
024	7	a https://doi.org/10.3389/fcimb.2020.5715782 DOI
040		a (SwePub)lu
041		a engb eng
042		9 SwePub
072	7	a art2 swepub-publicationtype
072	7	a ref2 swepub-contenttype
100	1	a Andersson, Tildeu Lund University,Lunds universitet,Experimental Infection Medicine,Forskargrupper vid Lunds universitet,Lund University Research Groups4 aut0 (Swepub:lu)ti3042an
245	1 0	a Development of a Molecular Imprinting-Based Surface Plasmon Resonance Biosensor for Rapid and Sensitive Detection of Staphylococcus aureus Alpha Hemolysin From Human Serum
264		c 2020-11-20
264	1	b Frontiers Media SA,c 2020
520		a Stapylococcus aureus is a common infectious agent in e.g. sepsis, associated with both high mortality rates and severe long-term effects. The cytolytic protein α-hemolysin has repeatedly been shown to enhance the virulence of S. aureus. Combined with an unhindered spread of multi drug-resistant strains, this has triggered research into novel anti virulence (i.e. anti α-hemolysin) drugs. Their functionality will depend on our ability to identify infections that might be alleviated by such. We therefore saw a need for detection methods that could identify individuals suffering from S. aureus infections where α-hemolysin was a major determinant. Molecular imprinted polymers were subsequently prepared on gold coated sensor chips. Used in combination with a surface plasmon resonance biosensor, α-hemolysin could therethrough be quantified from septic blood samples (n = 9), without pre-culturing of the infectious agent. The biosensor recognized α-hemolysin with high affinity (KD = 2.75 x 10-7 M) and demonstrated a statistically significant difference (p < 0.0001) between the α-hemolysin response and potential sample contaminants. The detection scheme proved equally good, or better, when compared to antibody-based detection methods. This novel detection scheme constitutes a more rapid, economical, and user-friendly alternative to many methods currently in use. Heightening both reproducibility and sensitivity, molecular imprinting in combination with surface plasmon resonance (SPR)-technology could be a versatile new tool in clinical- and research-settings alike.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Mikrobiologi inom det medicinska området0 (SwePub)301092 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Microbiology in the medical area0 (SwePub)301092 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Infektionsmedicin0 (SwePub)302092 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Infectious Medicine0 (SwePub)302092 hsv//eng
653		a alpha hemolysin
653		a molecular imprinting
653		a sepsis
653		a SPR
653		a Staphylococcus aureus
700	1	a Bläckberg, Annau Lund University,Lunds universitet,Translationell infektionsmedicin,Forskargrupper vid Lunds universitet,Translational infection medicine,Lund University Research Groups,Skåne University Hospital4 aut0 (Swepub:lu)an7442bl
700	1	a Lood, Rolfu Lund University,Lunds universitet,Experimental Infection Medicine,Forskargrupper vid Lunds universitet,Lund University Research Groups4 aut0 (Swepub:lu)med-rll
700	1	a Ertürk Bergdahl, Gizemu Lund University,Lunds universitet,Experimental Infection Medicine,Forskargrupper vid Lunds universitet,Lund University Research Groups4 aut0 (Swepub:lu)gi2856er
710	2	a Experimental Infection Medicineb Forskargrupper vid Lunds universitet4 org
773	0	t Frontiers in cellular and infection microbiologyd : Frontiers Media SAg 10q 10x 2235-2988
856	4	u http://dx.doi.org/10.3389/fcimb.2020.571578x freey FULLTEXT
856	4	u https://www.frontiersin.org/articles/10.3389/fcimb.2020.571578/pdf
856	4 8	u https://lup.lub.lu.se/record/32ae4433-593f-4fce-b497-44204f0fcb25
856	4 8	u https://doi.org/10.3389/fcimb.2020.571578

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Av författaren/redakt...: Andersson, Tilde; Bläckberg, Anna; Lood, Rolf; Ertürk Bergdahl, ...

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