The dynamics of the LPS triggered inflammatory response of murine microglia under different culture and in vivo conditions

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: WFRF:(Hedtjärn Maj 1973) > (2006) > The dynamics of the...

The dynamics of the LPS triggered inflammatory response of murine microglia under different culture and in vivo conditions

Lund, Sören (author)

Christensen, Kenneth Vielstad. (author)

Hedtjärn, Maj, 1973 (author): Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology

Mortensen, Anne Louise (author)

Hagberg, Henrik, 1955 (author): Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences

Falsig, Jeppe (author)

Hasseldam, Henrik (author)

Schrattenholz, André (author)

Pörzgen, Peter (author)

Leist, Marcel (author)

show less...

(creator_code:org_t)

2006
2006
English.
In: J Neuroimmunol. ; 180:1-2, s. 71-87

Related links:: https://gup.ub.gu.se...

Journal article (peer-reviewed)

Abstract Subject headings

Overall, the inflammatory potential of lipopolysaccharide (LPS) in vitro and in vivo was investigated using different omics technologies. We investigated the hippocampal response to intracerebroventricular (i.c.v) LPS in vivo, at both the transcriptional and protein level. Here, a time course analysis of interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) showed a sharp peak at 4 h and a return to baseline at 16 h. The expression of inflammatory mediators was not temporally correlated with expression of the microglia marker F4/80, which did not peak until 2 days after LPS injection. Of 480 inflammation-related genes present on a microarray, 29 transcripts were robustly up-regulated and 90% of them were also detected in LPS stimulated primary microglia (PM) cultures. Further in vitro to in vivo comparison showed that the counter regulation response observed in vivo was less evident in vitro, as transcript levels in PM decreased relatively little over 16 h. This apparent deficiency of homeostatic control of the innate immune response in cultures may also explain why a group of genes comprising tnf receptor associated factor-1, endothelin-1 and schlafen-1 were regulated strongly in vitro, but not in vivo. When the overall LPS-induced transcriptional response of PM was examined on a large Affymetrix chip, chemokines and cytokines constituted the most strongly regulated and largest groups. Interesting new microglia markers included interferon-induced protein with tetratricopeptide repeat (ifit), immune responsive gene-1 (irg-1) and thymidylate kinase family LPS-inducible member (tyki). The regulation of the former two was confirmed on the protein level in a proteomics study. Furthermore, conspicuous regulation of several gene clusters was identified, for instance that of genes pertaining to the extra-cellular matrix and enzymatic regulation thereof. Although most inflammatory genes induced in vitro were transferable to our in vivo model, the observed discrepancy for some genes potentially represents regulatory factors present in the central nervous system (CNS) but not in vitro.

Keyword

Animals
Animals
Newborn
Antigens
Differentiation/immunology/metabolism
Cells
Cultured
Chemokine CCL2/immunology/metabolism
Disease Models
Animal
Encephalitis/chemically induced/immunology/*physiopathology
Gene Expression/*drug effects/immunology
Gene Expression Profiling
Gliosis/chemically induced/immunology/*physiopathology
Hippocampus/drug effects/immunology/physiopathology
Inflammation Mediators/pharmacology
Injections
Intraventricular
Interleukin-6/immunology/metabolism
Lipopolysaccharides/*pharmacology
Male
Mice
Mice
Inbred C57BL
Microglia/*drug effects/immunology
Oligonucleotide Array Sequence Analysis
Proteomics
RNA
Messenger/drug effects/metabolism
Trans-Activation (Genetics)/*drug effects/immunology
Up-Regulation/drug effects/immunology

Publication and Content Type

ref (subject category)
art (subject category)

Find in a library

J Neuroimmunol (Search for host publication in LIBRIS)

To the university's database

Find more in SwePub

By the author/editor: Lund, Sören; Christensen, Ken ...; Hedtjärn, Maj, 1 ...; Mortensen, Anne ...; Hagberg, Henrik, ...; Falsig, Jeppe; show more...; Hasseldam, Henri ...; Schrattenholz, A ...; Pörzgen, Peter; Leist, Marcel; show less...

Articles in the publication: J Neuroimmunol

By the university: University of Gothenburg

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se