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Proximity-dependent...
Proximity-dependent initiation of hybridization chain reaction
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- Koos, Björn (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- Cane, Gaëlle (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- Grannas, Karin (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- Löf, Liza (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- Arngården, Linda (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- Heldin, Johan (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- Clausson, Carl-Magnus (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- Klaesson, Axel (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- Hirvonen, M Karoliina (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- de Oliveira, Felipe Marques Souza (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- Talibov, Vladimir O (author)
- Uppsala universitet,Biokemi
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- Pham, Nhan T (author)
- School of Biological Sciences and School of Biomedical Sciences, University of Edinburgh, UK
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- Auer, Manfred (author)
- School of Biological Sciences and School of Biomedical Sciences, University of Edinburgh, UK
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- Danielson, U Helena (author)
- Uppsala universitet,Biokemi
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- Haybaeck, Johannes (author)
- Institute of Pathology, Medical University of Graz, Austria
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- Kamali-Moghaddam, Masood (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- Söderberg, Ola (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- 2015-06-12
- 2015
- English.
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In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 6
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Abstract
Subject headings
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- Sensitive detection of protein interactions and post-translational modifications of native proteins is a challenge for research and diagnostic purposes. A method for this, which could be used in point-of-care devices and high-throughput screening, should be reliable, cost effective and robust. To achieve this, here we design a method (proxHCR) that combines the need for proximal binding with hybridization chain reaction (HCR) for signal amplification. When two oligonucleotide hairpins conjugated to antibodies bind in close proximity, they can be activated to reveal an initiator sequence. This starts a chain reaction of hybridization events between a pair of fluorophore-labelled oligonucleotide hairpins, generating a fluorescent product. In conclusion, we show the applicability of the proxHCR method for the detection of protein interactions and posttranslational modifications in microscopy and flow cytometry. As no enzymes are needed, proxHCR may be an inexpensive and robust alternative to proximity ligation assays.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Koos, Björn
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Cane, Gaëlle
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Grannas, Karin
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Löf, Liza
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Arngården, Linda
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Heldin, Johan
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show more...
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Clausson, Carl-M ...
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Klaesson, Axel
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Hirvonen, M Karo ...
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de Oliveira, Fel ...
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Talibov, Vladimi ...
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Pham, Nhan T
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Auer, Manfred
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Danielson, U Hel ...
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Haybaeck, Johann ...
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Kamali-Moghaddam ...
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Söderberg, Ola
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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Nature Communica ...
- By the university
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Uppsala University