A spectroscopic study of the photobleaching of protoporphyrin IX in solution

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Fältnamn	Indikatorer	Metadata
000		04204naa a2200529 4500
001		oai:gup.ub.gu.se/85068
003		SwePub
008		240528s2003 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://gup.ub.gu.se/publication/850682 URI
040		a (SwePub)gu
041		a eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Ericson, Marica B,d 1974u Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)4 aut0 (Swepub:gu)xemarq
245	1 0	a A spectroscopic study of the photobleaching of protoporphyrin IX in solution
264	1	c 2003
520		a Photodynamic therapy (PDT) has developed into an important new clinical treatment for cancer during the past 30 years. The method is non-invasive and based on the photochemical activity of a photosensitising agent present in cells and tissues. In so-called ALA-PDT, protoporphyrin IX (Pp IX) is induced from aminolaevulinic acid (ALA) applied topically or systemically. It has been shown that Pp IX is photodegraded by a photo-oxidation process and that its photoproducts have a characteristic absorption band around 670 nm, as observed both in solution and in cells incubated with ALA. In this study, the involvement of oxygen in the photobleaching process was verified by studying the effect of oxygen depletion using the freeze-pump-thaw (FPT) method. A solution of Pp IX in dimethylformamide (DMF) was exposed to light in the wavelength region 600-700 nm (peak centred at 620 (+/-25) nm) both in the presence and in the absence of oxygen. The bleaching process was observed by absorbance and fluorescence measurements. Photobleaching was observed in the presence of oxygen, as verified by the build-up of a photoproduct absorbing at 670 nm. When the sample was deoxygenated with the FPT method, the photoproduct absorption peak at 670 nm was missing. These results confirm that the formation of photo-protopor-phyrin is a photo-oxidation process and that no photobleaching takes place in the absence of oxygen. When comparing our results to the studies carried out by N-2 bubbling, the N-2 bubbling seems to be insufficient to remove the oxygen completely from the solution.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Dermatologi och venereologi0 (SwePub)302042 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Dermatology and Venereal Diseases0 (SwePub)302042 hsv//eng
650	7	a NATURVETENSKAPx Fysikx Atom- och molekylfysik och optik0 (SwePub)103022 hsv//swe
650	7	a NATURAL SCIENCESx Physical Sciencesx Atom and Molecular Physics and Optics0 (SwePub)103022 hsv//eng
653		a absorbance
653		a fluorescence
653		a oxygen
653		a photobleaching
653		a photodynamic therapy
653		a protoporphyrin IX
653		a basal-cell carcinoma
653		a hairless mouse skin
653		a photodynamic therapy
653		a 5-aminolevulinic acid
653		a endogenous porphyrins
653		a photofrin-ii
653		a light
653		a hematoporphyrin
653		a dosimetry
653		a phototransformations
700	1	a Grapengiesser, S.4 aut
700	1	a Gudmundson, F.4 aut
700	1	a Wennberg, Ann-Marie,d 1956u Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för dermatologi och venereologi,Institute of Selected Clinical Sciences, Department of Dermatology and Venereology4 aut0 (Swepub:gu)xwenna
700	1	a Larkö, Olle,d 1952u Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för dermatologi och venereologi,Institute of Selected Clinical Sciences, Department of Dermatology and Venereology4 aut0 (Swepub:gu)xlarol
700	1	a Moan, J.4 aut
700	1	a Rosén, A.u Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)4 aut
710	2	a Göteborgs universitetb Institutionen för fysik (GU)4 org
773	0	t Lasers in Medical Scienceg 18:1, s. 56-62q 18:1<56-62x 0268-8921
856	4 8	u https://gup.ub.gu.se/publication/85068

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