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Detecting harmful a...
Detecting harmful algal blooms with nucleic acid amplification-based biotechnological tools
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- Toldrà Filella, Anna (author)
- KTH,Fiberteknologi,IRTA, Ctra Poble Nou Km 5-5, Tarragona 43540, Spain.
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- O'Sullivan, Ciara K. (author)
- Univ Rovira & Virgili, Dept Engn Quim, Ave Paisos Catalans 26, Tarragona 43007, Spain.;Inst Catalana Recerca & Estudis Avancats ICREA, Pg Lluis Co 23, Barcelona 08010, Spain.
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- Diogene, Jorge (author)
- IRTA, Ctra Poble Nou Km 5-5, Tarragona 43540, Spain.
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- Campas, Monica (author)
- IRTA, Ctra Poble Nou Km 5-5, Tarragona 43540, Spain.
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(creator_code:org_t)
- Elsevier BV, 2020
- 2020
- English.
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In: Science of the Total Environment. - : Elsevier BV. - 0048-9697 .- 1879-1026. ; 749
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Harmful algal blooms (HABs) represent a growing threat to aquatic ecosystems and humans. Effective HAB management and mitigation efforts strongly rely on the availability of timely and in-situ tools for the detection of microalgae. In this sense, nudeic acid-based (molecular) methods are being considered for the unequivocal identification of miaoalgae as an attractive alternative to the currently used time-consuming and laboratory-based light microscopy techniques. This review provides an overview of the progress made on new molecular biotechnological tools for microalgal detection, particularly focusing on those that combine a nudeic acid (DNA or RNA) amplification step with detection. Different types of amplification processes (thermal and isothermal) and detection formats (e.g. microarrays, biosensors, lateral flows) are presented, and a comprehensive overview of their advantages and limitations is provided Although isothermal techniques are an attractive alternative to thermal amplification to reach in-situ analysis, further development is still required. Finally, current challenges, critical steps and future directions of the whole analysis process ( from sample procurement to in-situ implementation) are described.
Subject headings
- NATURVETENSKAP -- Biologi -- Ekologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Ecology (hsv//eng)
Keyword
- Harmful algal blooms (HABs)
- Microalgae
- Molecular method
- Isothermal DNA amplification
- In-situ HAB monitoring
Publication and Content Type
- ref (subject category)
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