Detecting harmful algal blooms with nucleic acid amplification-based biotechnological tools

Toldrà Filella, AnnaKTH,Fiberteknologi,IRTA, Ctra Poble Nou Km 5-5, Tarragona 43540, Spain. (author)

QC 20201218
Harmful algal blooms (HABs) represent a growing threat to aquatic ecosystems and humans. Effective HAB management and mitigation efforts strongly rely on the availability of timely and in-situ tools for the detection of microalgae. In this sense, nudeic acid-based (molecular) methods are being considered for the unequivocal identification of miaoalgae as an attractive alternative to the currently used time-consuming and laboratory-based light microscopy techniques. This review provides an overview of the progress made on new molecular biotechnological tools for microalgal detection, particularly focusing on those that combine a nudeic acid (DNA or RNA) amplification step with detection. Different types of amplification processes (thermal and isothermal) and detection formats (e.g. microarrays, biosensors, lateral flows) are presented, and a comprehensive overview of their advantages and limitations is provided Although isothermal techniques are an attractive alternative to thermal amplification to reach in-situ analysis, further development is still required. Finally, current challenges, critical steps and future directions of the whole analysis process ( from sample procurement to in-situ implementation) are described.

O'Sullivan, Ciara K.Univ Rovira & Virgili, Dept Engn Quim, Ave Paisos Catalans 26, Tarragona 43007, Spain.;Inst Catalana Recerca & Estudis Avancats ICREA, Pg Lluis Co 23, Barcelona 08010, Spain. (author)
Diogene, JorgeIRTA, Ctra Poble Nou Km 5-5, Tarragona 43540, Spain. (author)
Campas, MonicaIRTA, Ctra Poble Nou Km 5-5, Tarragona 43540, Spain. (author)
KTHFiberteknologi (creator_code:org_t)

By the author/editor: Toldrà Filella, ...; O'Sullivan, Ciar ...; Diogene, Jorge; Campas, Monica

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