WFRF:(O'Sullivan Anna)
Sökning: WFRF:(O'Sullivan Anna) > (2020) > Detecting harmful a...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03063naa a2200385 4500 | |
| 001 | oai:DiVA.org:kth-287520 | |
| 003 | SwePub | |
| 008 | 201218s2020 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2875202 URI |
| 024 | 7 | a https://doi.org/10.1016/j.scitotenv.2020.1416052 DOI |
| 040 | a (SwePub)kth | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a for2 swepub-publicationtype |
| 100 | 1 | a Toldrà Filella, Annau KTH,Fiberteknologi,IRTA, Ctra Poble Nou Km 5-5, Tarragona 43540, Spain.4 aut0 (Swepub:kth)u16e1g97 |
| 245 | 1 0 | a Detecting harmful algal blooms with nucleic acid amplification-based biotechnological tools |
| 264 | 1 | b Elsevier BV,c 2020 |
| 338 | a print2 rdacarrier | |
| 500 | a QC 20201218 | |
| 520 | a Harmful algal blooms (HABs) represent a growing threat to aquatic ecosystems and humans. Effective HAB management and mitigation efforts strongly rely on the availability of timely and in-situ tools for the detection of microalgae. In this sense, nudeic acid-based (molecular) methods are being considered for the unequivocal identification of miaoalgae as an attractive alternative to the currently used time-consuming and laboratory-based light microscopy techniques. This review provides an overview of the progress made on new molecular biotechnological tools for microalgal detection, particularly focusing on those that combine a nudeic acid (DNA or RNA) amplification step with detection. Different types of amplification processes (thermal and isothermal) and detection formats (e.g. microarrays, biosensors, lateral flows) are presented, and a comprehensive overview of their advantages and limitations is provided Although isothermal techniques are an attractive alternative to thermal amplification to reach in-situ analysis, further development is still required. Finally, current challenges, critical steps and future directions of the whole analysis process ( from sample procurement to in-situ implementation) are described. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Ekologi0 (SwePub)106112 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Ecology0 (SwePub)106112 hsv//eng |
| 653 | a Harmful algal blooms (HABs) | |
| 653 | a Microalgae | |
| 653 | a Molecular method | |
| 653 | a Isothermal DNA amplification | |
| 653 | a In-situ HAB monitoring | |
| 700 | 1 | a O'Sullivan, Ciara K.u Univ Rovira & Virgili, Dept Engn Quim, Ave Paisos Catalans 26, Tarragona 43007, Spain.;Inst Catalana Recerca & Estudis Avancats ICREA, Pg Lluis Co 23, Barcelona 08010, Spain.4 aut |
| 700 | 1 | a Diogene, Jorgeu IRTA, Ctra Poble Nou Km 5-5, Tarragona 43540, Spain.4 aut |
| 700 | 1 | a Campas, Monicau IRTA, Ctra Poble Nou Km 5-5, Tarragona 43540, Spain.4 aut |
| 710 | 2 | a KTHb Fiberteknologi4 org |
| 773 | 0 | t Science of the Total Environmentd : Elsevier BVg 749q 749x 0048-9697x 1879-1026 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-287520 |
| 856 | 4 8 | u https://doi.org/10.1016/j.scitotenv.2020.141605 |
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