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Transcriptomic resp...
Transcriptomic responses generated by hepatocarcinogens in a battery of liver-based in vitro models
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- Doktorova, Tatyana Y. (författare)
- Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Brussels, Belgium
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- Yildirimman, Reha (författare)
- Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany
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- Vinken, Mathieu (författare)
- Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Brussels, Belgium
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- Vilardell, Mireia (författare)
- Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany
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- Vanhaecke, Tamara (författare)
- Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Brussels, Belgium
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- Gmuender, Hans (författare)
- Genedata AG, Basel, Switzerland
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- Bort, Roque (författare)
- Unit of Experimental Hepathology, University Hospital La Fe Valencia, Spain / Faculty of Medicine, Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain
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- Brolen, Gabriella (författare)
- Cellectis, Göteborg, Sweden
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- Holmgren, Gustav (författare)
- Högskolan i Skövde,Institutionen för vård och natur,Forskningscentrum för Systembiologi,Cellectis, Göteborg, Sweden
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- Li, Ruoya (författare)
- Biopredic International, Rennes, France
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- Chesne, Christophe (författare)
- Biopredic International, Rennes, France
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- van Delft, Joost (författare)
- Department of Toxicogenomics, Maastricht University, Maastricht, The Netherlands
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- Kleinjans, Jos (författare)
- Department of Toxicogenomics, Maastricht University, Maastricht, The Netherlands
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- Castell, Jose (författare)
- Unit of Experimental Hepathology, University Hospital La Fe Valencia, Spain / Faculty of Medicine, Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain
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- Björquist, Petter (författare)
- Cellectis, Göteborg, Sweden
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- Herwig, Ralf (författare)
- Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany
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- Rogiers, Vera (författare)
- Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Brussels, Belgium
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(creator_code:org_t)
- 2013-02-07
- 2013
- Engelska.
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Ingår i: Carcinogenesis. - : Oxford University Press. - 0143-3334 .- 1460-2180. ; 34:6, s. 1393-1402
- Relaterad länk:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- As the conventional approach to assess the potential of a chemical to cause cancer in humans still includes the 2-year rodent carcinogenicity bioassay, development of alternative methodologies is needed. In the present study, the transcriptomics responses following exposure to genotoxic (GTX) and non-genotoxic (NGTX) hepatocarcinogens and non-carcinogens (NC) in five liver-based in vitro models, namely conventional and epigenetically stabilized cultures of primary rat hepatocytes, the human hepatoma-derived cell lines HepaRG and HepG2 and human embryonic stem cell-derived hepatocyte-like cells, are examined. For full characterization of the systems, several bioinformatics approaches are employed including gene-based, ConsensusPathDB-based and classification analysis. They provide convincingly similar outcomes, namely that upon exposure to carcinogens, the HepaRG generates a gene classifier (a gene classifier is defined as a selected set of characteristic gene signatures capable of distinguishing GTX, NGTX carcinogens and NC) able to discriminate the GTX carcinogens from the NGTX carcinogens and NC. The other in vitro models also yield cancer-relevant characteristic gene groups for the GTX exposure, but some genes are also deregulated by the NGTX carcinogens and NC. Irrespective of the tested in vitro model, the most uniformly expressed pathways following GTX exposure are the p53 and those that are subsequently induced. The NGTX carcinogens triggered no characteristic cancer-relevant gene profiles in all liver-based in vitro systems. In conclusion, liver-based in vitro models coupled with transcriptomics techniques, especially in the case when the HepaRG cell line is used, represent valuable tools for obtaining insight into the mechanism of action and identification of GTX carcinogens.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- Naturvetenskap
- Natural sciences
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Doktorova, Tatya ...
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Yildirimman, Reh ...
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Vinken, Mathieu
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Vilardell, Mirei ...
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Vanhaecke, Tamar ...
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Gmuender, Hans
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Bort, Roque
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Brolen, Gabriell ...
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Holmgren, Gustav
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Li, Ruoya
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Chesne, Christop ...
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van Delft, Joost
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Kleinjans, Jos
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Castell, Jose
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Björquist, Pette ...
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Herwig, Ralf
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Rogiers, Vera
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Carcinogenesis
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