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High prevalence of ...
High prevalence of cytomegalovirus infection in surgical intestinal specimens from infants with necrotizing enterocolitis and spontaneous intestinal perforation : A retrospective observational study
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- Omarsdottir, Soley (författare)
- Karolinska Institute, Department of Medicine, Center for Molecular Medicine, Exp Cardiovascular Research Unit and Department of Neurology
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- Agnarsdottir, Margret (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Casper, Charlotte (författare)
- Karolinska Institutet,Paul Sabatier University, Children's Hospital, Unit of Neonatology
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- Orrego, Abiel (författare)
- Karolinska Institute, Karolinska University Hospital, Departments of Clinical Pathology and Cytology
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- Vanpee, Mireille (författare)
- Karolinska Institutet,Karolinska Institute, Karolinska University Hospital, Women's and Children's Health
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- Rahbar, Afsar (författare)
- Karolinska Institutet,Karolinska Institute, Department of Medicine, Center for Molecular Medicine, Exp Cardiovascular Research Unit and Department of Neurology
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- Söderberg-Nauclér, Cecilia (författare)
- Karolinska Institute, Department of Medicine, Center for Molecular Medicine, Exp Cardiovascular Research Unit and Department of Neurology
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(creator_code:org_t)
- Elsevier BV, 2017
- 2017
- Engelska.
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Ingår i: Journal of Clinical Virology. - : Elsevier BV. - 1386-6532 .- 1873-5967. ; 93, s. 57-64
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Background: Necrotizing enterocolitis (NEC) is a severe, often fatal gastrointestinal emergency that predominantly affects preterm infants, and there is evidence that neonatal cytomegalovirus (CMV) infection may in some cases contribute to its pathogenesis.Objectives: This study aimed to evaluate the prevalence of CMV in infants with NEC.Study design: Seventy intestinal specimens from 61 infants with NEC, spontaneous intestinal perforation (SIP), or related surgical complications were collected at Karolinska University Hospital and Uppsala University Hospital, Sweden. Ten specimens from autopsied infants without bowel disease served as controls. Samples were analyzed for CMV immediate-early antigen (IEA), CMV late antigen (LA), 5-lipoxigenase (5LO) and CMV-DNA by immunohistochemistry (IHC) and in situ hybridization (ISH), respectively. In 10 index samples, CMV DNA was analyzed with Taqman PCR after laser capture microdissection (LCM) of cells positive for CMV IEA by IHC.Results: CMV IEA was detected by IHC in 57 (81%) and CMV LA in 45 (64%) of 70 intestinal specimens from index cases; 2 (20%) of 10 control specimens were positive for both antigens. 5LO was detected in intestinal tissue section obtained from all examined index and controls. CMV DNA was detected in 4 of 10 samples (40%) after LCM. By ISH, all 13 IHC-IEA-positive samples were positive for CMV DNA; however, 3 of 5 IHC-IEAnegative samples (60%) were also positive.Conclusions: CMV-specific antigens and CMV DNA were highly prevalent in intestinal specimens from infants with NEC, SIP, and related surgical complications. Our findings provide further evidence that neonatal CMV infection contributes to the pathogenesis of these diseases and may affect patient outcome.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- CMV
- Cytomegalovirus
- ELBW
- Extremely low birth weight
- NEC
- Necrotizing enterocolitis
- Spontaneous intestinal perforation
- VLBW
- Very low birth weight
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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