Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes

Bjelčić, Monika (författare): Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory

Sigfridsson Clauss, Kajsa G.V. (författare): Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory

Aurelius, Oskar (författare): Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory

Milas, Mirko (författare): Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory

Nan, Jie (författare): Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory

Ursby, Thomas (författare): Lund University,Lunds universitet,MAX IV-laboratoriet,LINXS - Institute of advanced Neutron and X-ray Science,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,MAX IV Laboratory,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH

(creator_code:org_t)

2023
2023
Engelska 8 s.
Ingår i: Acta Crystallographica Section D: Structural Biology. - 2059-7983. ; 79:Pt 11, s. 1018-1025

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Abstract Ämnesord

Stäng

In recent years, the emergence of serial crystallography, initially pioneered at X-ray free-electron lasers (XFELs), has sparked a growing interest in collecting macromolecular crystallographic data at room temperature. Various fixed-target serial crystallography techniques have been developed, ranging from commercially available chips to in-house designs implemented at different synchrotron facilities. Nevertheless, there is currently no commercially available chip (known to the authors) specifically designed for the direct handling of oxygen-sensitive samples. This study presents a methodology employing silicon nitride chips arranged in a 'sandwich' configuration, enabling reliable room-temperature data collection from oxygen-sensitive samples. The method involves the utilization of a custom-made 3D-printed assembling tool and a MX sample holder. To validate the effectiveness of the proposed method, deoxyhemoglobin and methemoglobin samples were investigated using the BioMAX X-ray macromolecular crystallography beamline, the Balder X-ray absorption spectroscopy beamline and UV-Vis absorption spectroscopy.

Av författaren/redakt...: Bjelčić, Monika; Sigfridsson Clau ...; Aurelius, Oskar; Milas, Mirko; Nan, Jie; Ursby, Thomas

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