Sökning: WFRF:(Truffer B.) > (2020-2024) > One-Tip enables com...
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001 | oai:DiVA.org:uu-530047 | |
003 | SwePub | |
008 | 240603s2024 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5300472 URI |
024 | 7 | a https://doi.org/10.1038/s41467-024-46777-92 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Ye, Ziluu Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Common Mech Res Major Dis, Suzhou Inst Syst Med, Suzhou, Peoples R China.;Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark.4 aut |
245 | 1 0 | a One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes |
264 | 1 | b Springer Nature,c 2024 |
338 | a electronic2 rdacarrier | |
500 | a De två första författarna delar förstaförfattarskapet | |
520 | a Mass spectrometry (MS)-based proteomics workflows typically involve complex, multi-step processes, presenting challenges with sample losses, reproducibility, requiring substantial time and financial investments, and specialized skills. Here we introduce One-Tip, a proteomics methodology that seamlessly integrates efficient, one-pot sample preparation with precise, narrow-window data-independent acquisition (nDIA) analysis. One-Tip substantially simplifies sample processing, enabling the reproducible identification of >9000 proteins from ~1000 HeLa cells. The versatility of One-Tip is highlighted by nDIA identification of ~6000 proteins in single cells from early mouse embryos. Additionally, the study incorporates the Uno Single Cell Dispenser™, demonstrating the capability of One-Tip in single-cell proteomics with >3000 proteins identified per HeLa cell. We also extend One-Tip workflow to analysis of extracellular vesicles (EVs) extracted from blood plasma, demonstrating its high sensitivity by identifying >3000 proteins from 16 ng EV preparation. One-Tip expands capabilities of proteomics, offering greater depth and throughput across a range of sample types. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
650 | 7 | a NATURVETENSKAPx Kemix Analytisk kemi0 (SwePub)104012 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Chemical Sciencesx Analytical Chemistry0 (SwePub)104012 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Immunologi inom det medicinska området0 (SwePub)301102 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Immunology in the medical area0 (SwePub)301102 hsv//eng |
700 | 1 | a Sabatier, Pierreu Uppsala universitet,Thoraxkirurgi,Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark4 aut0 (Swepub:uu)piesa271 |
700 | 1 | a Martin-Gonzalez, Javieru Univ Copenhagen, Dept Expt Med, Core Facil Transgenic Mice, Copenhagen, Denmark.4 aut |
700 | 1 | a Eguchi, Akihirou Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark.4 aut |
700 | 1 | a Lechner, Maicou Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark.4 aut |
700 | 1 | a Østergaard, Oleu Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark.4 aut |
700 | 1 | a Xie, Jingshengu Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Common Mech Res Major Dis, Suzhou Inst Syst Med, Suzhou, Peoples R China.4 aut |
700 | 1 | a Guo, Yuanu Tecan Grp Ltd, Mannedorf, Switzerland.4 aut |
700 | 1 | a Schultz, Lesleyu Tecan Grp Ltd, Mannedorf, Switzerland.4 aut |
700 | 1 | a Truffer, Rafaelau Tecan Grp Ltd, Mannedorf, Switzerland.4 aut |
700 | 1 | a Bekker-Jensen, Dorte B.u Evosep Biosyst, Odense, Denmark.4 aut |
700 | 1 | a Bache, Nicolaiu Evosep Biosyst, Odense, Denmark.4 aut |
700 | 1 | a Olsen, Jesper V.u Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark.4 aut |
710 | 2 | a Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Common Mech Res Major Dis, Suzhou Inst Syst Med, Suzhou, Peoples R China.;Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark.b Thoraxkirurgi4 org |
773 | 0 | t Nature Communicationsd : Springer Natureg 15:1q 15:1x 2041-1723 |
856 | 4 | u https://doi.org/10.1038/s41467-024-46777-9y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1864528/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-530047 |
856 | 4 8 | u https://doi.org/10.1038/s41467-024-46777-9 |
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