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Sökning: WFRF:(Truffer B.) > (2020-2024) > One-Tip enables com...

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FältnamnIndikatorerMetadata
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001oai:DiVA.org:uu-530047
003SwePub
008240603s2024 | |||||||||||000 ||eng|
024a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5300472 URI
024a https://doi.org/10.1038/s41467-024-46777-92 DOI
040 a (SwePub)uu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
100a Ye, Ziluu Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Common Mech Res Major Dis, Suzhou Inst Syst Med, Suzhou, Peoples R China.;Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark.4 aut
2451 0a One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes
264 1b Springer Nature,c 2024
338 a electronic2 rdacarrier
500 a De två första författarna delar förstaförfattarskapet
520 a Mass spectrometry (MS)-based proteomics workflows typically involve complex, multi-step processes, presenting challenges with sample losses, reproducibility, requiring substantial time and financial investments, and specialized skills. Here we introduce One-Tip, a proteomics methodology that seamlessly integrates efficient, one-pot sample preparation with precise, narrow-window data-independent acquisition (nDIA) analysis. One-Tip substantially simplifies sample processing, enabling the reproducible identification of >9000 proteins from ~1000 HeLa cells. The versatility of One-Tip is highlighted by nDIA identification of ~6000 proteins in single cells from early mouse embryos. Additionally, the study incorporates the Uno Single Cell Dispenser™, demonstrating the capability of One-Tip in single-cell proteomics with >3000 proteins identified per HeLa cell. We also extend One-Tip workflow to analysis of extracellular vesicles (EVs) extracted from blood plasma, demonstrating its high sensitivity by identifying >3000 proteins from 16 ng EV preparation. One-Tip expands capabilities of proteomics, offering greater depth and throughput across a range of sample types.
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
650 7a NATURVETENSKAPx Kemix Analytisk kemi0 (SwePub)104012 hsv//swe
650 7a NATURAL SCIENCESx Chemical Sciencesx Analytical Chemistry0 (SwePub)104012 hsv//eng
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Immunologi inom det medicinska området0 (SwePub)301102 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Immunology in the medical area0 (SwePub)301102 hsv//eng
700a Sabatier, Pierreu Uppsala universitet,Thoraxkirurgi,Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark4 aut0 (Swepub:uu)piesa271
700a Martin-Gonzalez, Javieru Univ Copenhagen, Dept Expt Med, Core Facil Transgenic Mice, Copenhagen, Denmark.4 aut
700a Eguchi, Akihirou Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark.4 aut
700a Lechner, Maicou Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark.4 aut
700a Østergaard, Oleu Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark.4 aut
700a Xie, Jingshengu Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Common Mech Res Major Dis, Suzhou Inst Syst Med, Suzhou, Peoples R China.4 aut
700a Guo, Yuanu Tecan Grp Ltd, Mannedorf, Switzerland.4 aut
700a Schultz, Lesleyu Tecan Grp Ltd, Mannedorf, Switzerland.4 aut
700a Truffer, Rafaelau Tecan Grp Ltd, Mannedorf, Switzerland.4 aut
700a Bekker-Jensen, Dorte B.u Evosep Biosyst, Odense, Denmark.4 aut
700a Bache, Nicolaiu Evosep Biosyst, Odense, Denmark.4 aut
700a Olsen, Jesper V.u Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark.4 aut
710a Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Common Mech Res Major Dis, Suzhou Inst Syst Med, Suzhou, Peoples R China.;Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark.b Thoraxkirurgi4 org
773t Nature Communicationsd : Springer Natureg 15:1q 15:1x 2041-1723
856u https://doi.org/10.1038/s41467-024-46777-9y Fulltext
856u https://uu.diva-portal.org/smash/get/diva2:1864528/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-530047
8564 8u https://doi.org/10.1038/s41467-024-46777-9

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