Sökning: WFRF:(Vanselow Katja) > Novel asymmetricall...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 03763naa a2200589 4500 | |
001 | oai:DiVA.org:kth-33978 | |
003 | SwePub | |
008 | 110523s2011 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-339782 URI |
024 | 7 | a https://doi.org/10.1038/emboj.2011.632 DOI |
040 | a (SwePub)kth | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Jakobsen, Lis4 aut |
245 | 1 0 | a Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods |
264 | c 2011-03-11 | |
264 | 1 | b Wiley,c 2011 |
338 | a print2 rdacarrier | |
500 | a QC 20110530 | |
520 | a Centrosomes in animal cells are dynamic organelles with a proteinaceous matrix of pericentriolar material assembled around a pair of centrioles. They organize the microtubule cytoskeleton and the mitotic spindle apparatus. Mature centrioles are essential for biogenesis of primary cilia that mediate key signalling events. Despite recent advances, the molecular basis for the plethora of processes coordinated by centrosomes is not fully understood. We have combined protein identification and localization, using PCP-SILAC mass spectrometry, BAC transgeneOmics, and antibodies to define the constituents of human centrosomes. From a background of non-specific proteins, we distinguished 126 known and 40 candidate centrosomal proteins, of which 22 were confirmed as novel components. An antibody screen covering 4000 genes revealed an additional 113 candidates. We illustrate the power of our methods by identifying a novel set of five proteins preferentially associated with mother or daughter centrioles, comprising genes implicated in cell polarity. Pulsed labelling demonstrates a remarkable variation in the stability of centrosomal protein complexes. These spatiotemporal proteomics data provide leads to the further functional characterization of centrosomal proteins. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Medicinsk bioteknologi0 (SwePub)304012 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Medical Biotechnology0 (SwePub)304012 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
653 | a centrosome | |
653 | a mass spectrometry-based proteomics | |
653 | a mother and daughter centriole | |
653 | a protein turnover | |
653 | a SILAC | |
653 | a Biochemistry | |
653 | a Biokemi | |
653 | a Molecular biology | |
653 | a Molekylärbiologi | |
700 | 1 | a Vanselow, Katja4 aut |
700 | 1 | a Skogs, Marieu KTH,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1hik3sg |
700 | 1 | a Toyoda, Yusuke4 aut |
700 | 1 | a Lundberg, Emmau KTH,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u12bylj1 |
700 | 1 | a Poser, Ina4 aut |
700 | 1 | a Falkenby, Lasse G.4 aut |
700 | 1 | a Bennetzen, Martin4 aut |
700 | 1 | a Westendorf, Jens4 aut |
700 | 1 | a Nigg, Erich A.4 aut |
700 | 1 | a Uhlen, Mathiasu KTH,Proteomik4 aut0 (Swepub:kth)u1dulvmw |
700 | 1 | a Hyman, Anthony A.4 aut |
700 | 1 | a Andersen, Jens S.4 aut |
710 | 2 | a KTHb Science for Life Laboratory, SciLifeLab4 org |
773 | 0 | t EMBO Journald : Wileyg 30:8, s. 1520-1535q 30:8<1520-1535x 0261-4189x 1460-2075 |
856 | 4 | u https://europepmc.org/articles/pmc3102290?pdf=render |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-33978 |
856 | 4 8 | u https://doi.org/10.1038/emboj.2011.63 |
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