The in silico identification and characterization of a bread wheat/Triticum militinae introgression line: Characterization of alien introgression in wheat

The in silico identification and characterization of a bread wheat/Triticum militinae introgression line : Characterization of alien introgression in wheat

Abrouk, Michael (författare): Institute of Experimental Botany, Centre of the Region Hana for Biotechnological and Agricultural Research, Olomouc, Czech Republic

Balcarkova, Barbora (författare): Institute of Experimental Botany, Centre of the Region Hana for Biotechnological and Agricultural Research, Olomouc, Czech Republic

Simkova, Hana (författare): Institute of Experimental Botany, Centre of the Region Hana for Biotechnological and Agricultural Research, Olomouc, Czech Republic

Kominkova, Eva (författare): Institute of Experimental Botany, Centre of the Region Hana for Biotechnological and Agricultural Research, Olomouc, Czech Republic

Martis, Mihaela-Maria, 1979- (författare): Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten,Institute for Bioinformatics and Systems Biology, Helmholtz Center Munich, Neuherberg, Germany

Jakobson, Irena (författare): Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, Tallinn 19086, Estonia

Timofejeva, Ljudmilla (författare): Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, Tallinn 19086, Estonia

Rey, Elodie (författare): Institute of Experimental Botany, Centre of the Region Hana for Biotechnological and Agricultural Research, Olomouc, Czech Republic

Vrana, Jan (författare): Institute of Experimental Botany, Centre of the Region Hana for Biotechnological and Agricultural Research, Olomouc, Czech Republic

Kilian, Andrzej (författare): Diversity Arrays Technology Pty Ltd, Yarralumla, ACT2600, Australia

Järve, Kadri (författare): Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, Tallinn 19086, Estonia

Dolezel, Jaroslav (författare): Institute of Experimental Botany, Centre of the Region Hana for Biotechnological and Agricultural Research, Olomouc, Czech Republic

Valarik, Miroslav (författare): Institute of Experimental Botany, Centre of the Region Hana for Biotechnological and Agricultural Research, Olomouc, Czech Republic

(creator_code:org_t)

2016-09-16
2017
Engelska.
Ingår i: Plant Biotechnology Journal. - : John Wiley & Sons. - 1467-7644 .- 1467-7652. ; 15:2, s. 249-256

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Abstract Ämnesord

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The capacity of the bread wheat (Triticum aestivum) genome to tolerate introgression from related genomes can be exploited for wheat improvement. A resistance to powdery mildew expressed by a derivative of the cross bread wheat cv. Tähti ⨯ T. militinae (Tm) is known to be due to the incorporation of a Tm segment into the long arm of chromosome 4A. Here, a newly developed in silico method termed RICh (rearrangement identification and characterization) has been applied to characterize the introgression. A virtual gene order, assembled using the GenomeZipper approach, was obtained for the native copy of chromosome 4A; it incorporated 570 4A DArTseq markers to produce a zipper comprising 2,132 loci. A comparison between the native and introgressed forms of the 4AL chromosome arm showed that the introgressed region is located at the distal part of the arm. The Tm segment, derived from chromosome 7G, harbors 131 homoeologs out of the 357 genes present on the corresponding region of Chinese Spring 4AL. The estimated number of Tm genes transferred along with the disease resistance gene was 169. Characterizing the introgression's position, gene content and internal gene order should facilitate not only gene isolation, but may also be informative with respect to chromatin structure and behavior studies.

Av författaren/redakt...: Abrouk, Michael; Balcarkova, Barb ...; Simkova, Hana; Kominkova, Eva; Martis, Mihaela- ...; Jakobson, Irena; visa fler...; Timofejeva, Ljud ...; Rey, Elodie; Vrana, Jan; Kilian, Andrzej; Järve, Kadri; Dolezel, Jarosla ...; Valarik, Mirosla ...; visa färre...

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