WFRF:(Andersen Mikkel R.)
Search: WFRF:(Andersen Mikkel R.) > Results from the fi...
- 20 of 22
- Previous record
- Next record
- To hitlist
- Jensen, Christian Fuglesang S.University of Copenhagen,Gentofte Hospital (author)
Results from the first autologous grafting of adult human testis tissue : A case report
- Article/chapterEnglish2024
Publisher, publication year, extent ...
- 2024
- 7 s.
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:62664409-e7bf-4cbf-a647-6232631338c4
- https://lup.lub.lu.se/record/62664409-e7bf-4cbf-a647-6232631338c4URI
- https://doi.org/10.1093/humrep/dead243DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Fertility restoration using autologous testicular tissue transplantation is relevant for infertile men surviving from childhood cancer and, possibly, in men with absent or incomplete spermatogenesis resulting in the lack of spermatozoa in the ejaculate (non-obstructive azoospermia, NOA). Currently, testicular tissue from pre-pubertal boys extracted before treatment with gonadotoxic cancer therapy can be cryopreserved with good survival of spermatogonial stem cells. However, strategies for fertility restoration, after successful cancer treatment, are still experimental and no clinical methods have yet been developed. Similarly, no clinically available treatments can help men with NOA to become biological fathers after failed attempts of testicular surgical sperm retrieval. We present a case of a 31-year-old man with NOA who had three pieces of testis tissue (each ∼2 × 4 × 2 mm3) extracted and cryopreserved in relation to performing microdissection testicular sperm extraction (mTESE). Approximately 2 years after mTESE, the thawed tissue pieces were engrafted in surgically created pockets bilaterally under the scrotal skin. Follow-up was performed after 2, 4, and 6 months with assessment of reproductive hormones and ultrasound of the scrotum. After 6 months, all engrafted tissue was extracted and microscopically analyzed for the presence of spermatozoa. Furthermore, parts of the extracted tissue were analyzed histologically and by immunohistochemical analysis. Active blood flow in the engrafted tissue was demonstrated by doppler ultrasound after 6 months. No spermatozoa were found in the extracted tissue. Histological and immunohistochemical analysis demonstrated graft survival with intact clear tubules and normal cell organization. Sertoli cells and spermatocytes with normal morphology were located near the basement membrane. MAGE-A and VASA positive spermatogonia/spermatocytes were detected together with SOX9 positive Sertoli cells. Spermatocytes and/or Sertoli cells positive for γH2AX was also detected. In summary, following autologous grafting of frozen-thawed testis tissue under the scrotal skin in a man with NOA, we demonstrated graft survival after 6 months. No mature spermatozoa were detected; however, this is likely due to the pre-existing spermatogenic failure.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Reproduktionsmedicin och gynekologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Obstetrics, Gynaecology and Reproductive Medicine hsv//eng
- fertility restoration
- male infertility
- non-obstructive azoospermia
- spermatogonial stem cells
- testis tissue
Added entries (persons, corporate bodies, meetings, titles ...)
- Mamsen, Linn SaltoCopenhagen University Hospital (author)
- Wang, DanyangUniversity of Copenhagen,Copenhagen University Hospital (author)
- Fode, MikkelGentofte Hospital,University of Copenhagen (author)
- Giwercman, AleksanderLund University,Lunds universitet,Reproduktionsmedicin, Malmö,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Reproductive medicine, Malmö,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Skåne University Hospital(Swepub:lu)kir-agi (author)
- Jørgensen, NielsCopenhagen University Hospital (author)
- Ohl, Dana A.University of Michigan (author)
- Fedder, JensOdense University Hospital (author)
- Hoffmann, Eva R.University of Copenhagen (author)
- Yding Andersen, ClausUniversity of Copenhagen,Copenhagen University Hospital (author)
- Sønksen, JensUniversity of Copenhagen,Gentofte Hospital (author)
- University of CopenhagenGentofte Hospital (creator_code:org_t)
Related titles
- In:Human Reproduction39:2, s. 303-3090268-1161
Internet link
Find in a library
- Human Reproduction (Search for host publication in LIBRIS)
To the university's database
- 20 of 22
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Jensen, Christia ...
- Mamsen, Linn Sal ...
- Wang, Danyang
- Fode, Mikkel
- Giwercman, Aleks ...
- Jørgensen, Niels
- show more...
- Ohl, Dana A.
- Fedder, Jens
- Hoffmann, Eva R.
- Yding Andersen, ...
- Sønksen, Jens
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Clinical Medicin ...
- and Obstetrics Gynae ...
- Articles in the publication
- Human Reproducti ...
- By the university
- Lund University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
