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  • Roepstorff, P.Department of Molecular Biology, Odense University, Denmark (author)

Application of Plasma Desorption Mass Spectrometry to Molecular Weight Determination of Structural Protein from Insect Cuticle

  • Article/chapterEnglish1986

Publisher, publication year, extent ...

  • Wiley,1986
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:kau-16970
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-16970URI
  • https://doi.org/10.1002/bms.1200131209DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • During sequence determination of a structural protein from the cuticle of the migratory locust, Locusta migratoria, a discrepancy was found between the molecular weight calculated from the sequence data (15 323) and that estimated by SDS-gel electrophoresis (21 600). The protein contains several repeated sequences, and the discrepancy might indicate that part of the sequence was missing or that the protein contained a large prosthetic group. The molecular weight of the protein was determined by 127I-plasma desorption mass spectrometry to be 15 329±50 which confirms the sequence data and shows the molecular weight determination based on SDS-electrophoresis to be too high.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Højrup, P.Department of Molecular Biology, Odense University, Denmark (author)
  • Sundqvist, B.U.R.Tandem Accelerator Laboratory, Uppsala University (author)
  • Jonsson, GunnarTandem Accelerator Laboratory, Uppsala University(Swepub:kau)gunnjons (author)
  • Håkansson, P.Tandem Accelerator Laboratory, Uppsala University (author)
  • Andersson, S. O.Institute for Biological Chemistry A, University of Copenhagen, Denmark (author)
  • Johansson, K. E.Mycoplasma Laboratory, The National Veterinary Institute, Uppsala (author)
  • Department of Molecular Biology, Odense University, DenmarkTandem Accelerator Laboratory, Uppsala University (creator_code:org_t)

Related titles

  • In:Journal of Mass Spectrometry: Wiley13:12, s. 689-6911076-51741096-9888
  • In:Biological Mass Spectrometry: Wiley13:12, s. 689-6911052-9306

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