Application of Plasma Desorption Mass Spectrometry to Molecular Weight Determination of Structural Protein from Insect Cuticle

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Fältnamn	Indikatorer	Metadata
000		02708naa a2200361 4500
001		oai:DiVA.org:kau-16970
003		SwePub
008		130121s1986 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-169702 URI
024	7	a https://doi.org/10.1002/bms.12001312092 DOI
040		a (SwePub)kau
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Roepstorff, P.u Department of Molecular Biology, Odense University, Denmark4 aut
245	1 0	a Application of Plasma Desorption Mass Spectrometry to Molecular Weight Determination of Structural Protein from Insect Cuticle
264	1	b Wiley,c 1986
338		a print2 rdacarrier
520		a During sequence determination of a structural protein from the cuticle of the migratory locust, Locusta migratoria, a discrepancy was found between the molecular weight calculated from the sequence data (15 323) and that estimated by SDS-gel electrophoresis (21 600). The protein contains several repeated sequences, and the discrepancy might indicate that part of the sequence was missing or that the protein contained a large prosthetic group. The molecular weight of the protein was determined by 127I-plasma desorption mass spectrometry to be 15 329±50 which confirms the sequence data and shows the molecular weight determination based on SDS-electrophoresis to be too high.
650	7	a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
700	1	a Højrup, P.u Department of Molecular Biology, Odense University, Denmark4 aut
700	1	a Sundqvist, B.U.R.u Tandem Accelerator Laboratory, Uppsala University4 aut
700	1	a Jonsson, Gunnaru Tandem Accelerator Laboratory, Uppsala University4 aut0 (Swepub:kau)gunnjons
700	1	a Håkansson, P.u Tandem Accelerator Laboratory, Uppsala University4 aut
700	1	a Andersson, S. O.u Institute for Biological Chemistry A, University of Copenhagen, Denmark4 aut
700	1	a Johansson, K. E.u Mycoplasma Laboratory, The National Veterinary Institute, Uppsala4 aut
710	2	a Department of Molecular Biology, Odense University, Denmarkb Tandem Accelerator Laboratory, Uppsala University4 org
773	0	t Journal of Mass Spectrometryd : Wileyg 13:12, s. 689-691q 13:12<689-691x 1076-5174x 1096-9888
773	0	t Biological Mass Spectrometryd : Wileyg 13:12, s. 689-691q 13:12<689-691x 1052-9306
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-16970
856	4 8	u https://doi.org/10.1002/bms.1200131209

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Av författaren/redakt...: Roepstorff, P.; Højrup, P.; Sundqvist, B.U.R ...; Jonsson, Gunnar; Håkansson, P.; Andersson, S. O.; visa fler...; Johansson, K. E.; visa färre...

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