Cloning and functional expression of the guinea pig neuropeptide Y Y2 receptor

Five neuropeptide Y (NPY) receptor subtypes have been cloned in mammals. The degree of sequence conservation differs considerably between subtypes as well as between evolutionary lineages. To shed further light on this, we have cloned the five NPY receptors in the guinea pig. Here, we report the cloning of the guinea pig Y2 receptor. The Y2 receptor is generally highly conserved, with 90-95% identity between different orders of mammals, including the guinea pig. The guinea pig receptor has a divergent cytoplasmic tail, indicating possible differences in regulation of signalling and/or down regulation. COS-7 cells transiently transfected with the gpY2 receptor show saturable 125I-PYY binding with a Kd = 6 pM. In displacement experiments, the gpY2 receptor was similar to the human and rat receptors with the following rank order of potencies: pNPY > pPYY > pNPY13-36 = pNPY22-36 > [Leu31Pro34]NPY > BIBP3226. Thus, the guinea pig Y2 receptor is well conserved in comparison with human and rat with regard to both amino acid sequence and pharmacological profile.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Farmakologi och toxikologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Pharmacology and Toxicology hsv//eng
Amino Acid Sequence
Animals
Arginine/analogs & derivatives
COS Cells
Cloning, Molecular
Conserved Sequence
Gene Expression
Guinea Pigs
Humans
In Vitro Techniques
Kinetics
Molecular Sequence Data
Neuropeptide Y/analogs & derivatives
Peptide Fragments/metabolism
Peptide YY/metabolism
Rats
Receptors, Neuropeptide Y/chemistry
Recombinant Proteins/chemistry
Sequence Homology, Amino Acid
Transfection

Holmberg, S KUppsala University (author)
Eriksson, HUppsala University (author)
Beck-Sickinger, A GETH Zürich (author)
Grundemar, LLund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)med-lgd (author)
Larhammar, DUppsala University (author)
Uppsala UniversityETH Zürich (creator_code:org_t)

By the author/editor: Sharma, P; Holmberg, S K; Eriksson, H; Beck-Sickinger, ...; Grundemar, L; Larhammar, D

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Pharmacology and ...

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