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B cells in tumor dr...
B cells in tumor draining lymph nodes act asefficient antigen presenting cells in cancer patients
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Zirakzadeh, A. Ali (författare)
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Krantz, David (författare)
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Winerdal, Malin (författare)
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Lundgren, Christian (författare)
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Hartana, Ciputra (författare)
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Ahlén Bergman, Emma (författare)
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Hansson, Johan (författare)
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Holmström, Benny (författare)
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Sidiki, Alexander (författare)
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- Vasko, Janos (författare)
- Umeå universitet,Patologi
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Johansson, Markus (författare)
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Marits, Per (författare)
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- Sherif, Amir (författare)
- Umeå universitet,Urologi och andrologi
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Winqvist, Ola (författare)
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(creator_code:org_t)
- BioMed Central, 2015
- 2015
- Engelska.
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Ingår i: Journal for ImmunoTherapy of Cancer. - : BioMed Central. - 2051-1426. ; 3:Suppl 2
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Introduction: Overall Survival of patients with muscle invasive urothelial bladder cancer MIBC remains around 50% (5 years), albeit some improvements by combining neoadjuvant chemotherapy with radical surgery. Our previous work has demonstrated that in vitro expansions of sentinel node-acquired autologous tumor specific CD4+ T cells are promising for adoptive immunotherapy [1]. In order for naive T helper cells to become activated, they need effective APCs, presenting tumor antigens. In another study, we observed that B cells in cancer patients were tumor antigen experienced and from their phenotypes we suggested a CD4+ T cell dependent anti-tumoral response [2]. In this study, we report a flow cytometric investigation of tumor draining lymph node (sentinel node) derived B cell activation by autologous tumor extract in patients with MIBC.Methods: Sentinel nodes (SNs) from 28 patients with MIBC were detected by a Geiger meter at cystectomy after peritumoral injection with radioactive isotope. Lymphocytes were isolated from freshly received SNs where they were stimulated with autologous tumor extract in a sterile environment. After cultivation for 7 days, the cells were analyzed by multi-color flow cytometry using FASCIA (Flow cytometric Assay of Specific Cell-mediated Immune response in Activated whole blood).Results: Patients displayed an increased B cell activation in SNs after stimulation with autologous tumor extract compared to when SN acquired lymphocytes were stimulated with autologous extract of macroscopically non-malignant bladder. CD4+ T cells from SNs were activated and formed blasts after co-culture with SN acquired B cells in the presence of tumor antigen. However, CD4+ T cells were not activated and did not blast when co-cultured with B cells incubated with HLA-DR-blocking antibodies. This indicates antigen presenting ability of SN acquired B cells.Conclusions: We demonstrate sentinel node acquired B lymphocytes can be activated in culture upon stimulation with autologous tumor extract but not with extract of non-malignant epithelium of the bladder, after 7 days. Lower number of sentinel node acquired CD4+ T cells cultured with HLA-DR blocked CD19+ cells in presence of tumor antigen, indicate functional antigen presenting ability of B cells in sentinel nodes. The role of B cells as APCs in human T cell anti-tumoral response should be further explored, as well as their usefulness in adoptive immunotherapy.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Urologi och njurmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Urology and Nephrology (hsv//eng)
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Zirakzadeh, A. A ...
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Krantz, David
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Winerdal, Malin
-
Lundgren, Christ ...
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Hartana, Ciputra
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Ahlén Bergman, E ...
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visa fler...
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Hansson, Johan
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Holmström, Benny
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Sidiki, Alexande ...
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Vasko, Janos
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Johansson, Marku ...
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Marits, Per
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Sherif, Amir
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Winqvist, Ola
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visa färre...
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